FastQCFastQC Report
Thu 26 May 2016
SRR1512546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512546_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590789
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47020.29557659752487603No Hit
CTGTAGGACGTGGAATATGGCAAGA44630.28055260628530876No Hit
GTCCTACAGTGGACATTTCTAAATT44420.27923250663664384No Hit
CTTTAGGACGTGAAATATGGCGAGG41490.26081397344336676No Hit
GTCCTAAAGTGTGTATTTCTCATTT41380.26012249267501847No Hit
TATCAACGCAGAGTACTTTTTTTTT33280.20920436336937204No Hit
GGTATCAACGCAGAGTACTTTTTTT32320.20316962211833245No Hit
GTACATGGGAAGCAGTGGTATCAAC26720.16796696482060158No Hit
GTCCTACAGTGTGCATTTCTCATTT26140.1643209753147652No Hit
CTGAAGGACCTGGAATATGGCGAGA23050.14489665191298154No Hit
CTGTAGGACCTGGAATATGGCGAGA22810.14338796660022166No Hit
TTTCTAAATTTTCCACCTTTTTCAG18380.11554015020219525No Hit
ATTTAGAAATGTCCACTGTAGGACG17870.11233419391258048No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15910.10001326385837468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8500.012.9678824
TAAGACG604.0836714E-412.669774
TAGAAAT6650.011.8600474
CCACCTT6850.011.37075713
AATGTCC6500.011.1072698
CTGTTCG600.005877424511.0829129
GGACCTG16850.010.8826026
GTCTTAC1752.0008883E-1110.868691
AGGACCT17400.010.8133335
GTTCTAA1605.347829E-1010.6988671
GTACTAG803.7339184E-410.6988671
ACCTTTT7400.010.65265615
TAAGACA2700.010.5581424
AATCCCG1355.7121724E-810.55216819
TGTAGGA27450.010.4542912
TGTCCAC7100.010.30210110
GACGTGG12450.010.3014617
CTGTAGG27650.010.2495871
GTAGGAC27450.010.246593
GGCGAGG11150.010.13575919
Produced by FastQC (version 0.11.5)