##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512546_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1590789 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89050779204533 31.0 31.0 34.0 28.0 34.0 2 31.20431936605043 31.0 31.0 34.0 28.0 34.0 3 31.377215960130478 33.0 31.0 34.0 28.0 34.0 4 34.96026814366959 37.0 35.0 37.0 32.0 37.0 5 34.27934125770294 35.0 35.0 37.0 31.0 37.0 6 34.605260659961814 37.0 35.0 37.0 32.0 37.0 7 34.58941317798904 37.0 35.0 37.0 32.0 37.0 8 34.575708029160374 37.0 35.0 37.0 32.0 37.0 9 36.146248182505666 39.0 37.0 39.0 32.0 39.0 10 35.99346676397687 39.0 37.0 39.0 32.0 39.0 11 36.058784037355046 39.0 37.0 39.0 32.0 39.0 12 35.96746771570585 39.0 37.0 39.0 32.0 39.0 13 35.943503506750424 39.0 37.0 39.0 31.0 39.0 14 37.11258815594023 40.0 38.0 41.0 31.0 41.0 15 37.08383135664126 40.0 37.0 41.0 31.0 41.0 16 37.030232167811064 40.0 37.0 41.0 31.0 41.0 17 36.98714977284857 40.0 37.0 41.0 31.0 41.0 18 36.92273016723148 40.0 37.0 41.0 31.0 41.0 19 36.95020898434676 40.0 37.0 41.0 31.0 41.0 20 36.92376298805184 40.0 37.0 41.0 31.0 41.0 21 36.86883929924082 40.0 37.0 41.0 31.0 41.0 22 36.7901387299007 40.0 37.0 41.0 31.0 41.0 23 36.703848844818516 40.0 37.0 41.0 31.0 41.0 24 36.63981772566947 40.0 37.0 41.0 31.0 41.0 25 36.544278971001184 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39345460351461625 1101 2 0.41592977706221745 1101 3 0.42516075531925424 1101 4 0.46864280661170454 1101 5 0.043275905914569535 1101 6 0.4037570892826423 1101 7 0.4647707215195638 1101 8 0.6018996960972416 1101 9 0.7168711604650042 1101 10 0.5960757023090082 1101 11 0.6995390208011116 1101 12 0.6867680143384192 1101 13 0.8136286577877456 1101 14 0.8928581138694653 1101 15 0.8359172179636829 1101 16 0.7973551688101352 1101 17 0.6776743349170928 1101 18 0.6008088512607301 1101 19 0.7795963613808894 1101 20 0.7774681361704197 1101 21 0.6219178335236748 1101 22 0.7933097615338554 1101 23 0.7275305458556005 1101 24 0.7531616880528489 1101 25 0.9004808478379189 1102 1 0.22148739058502542 1102 2 0.47579731705680217 1102 3 0.5323184976333017 1102 4 0.5376596575936361 1102 5 0.11015574651980131 1102 6 0.4898041358898766 1102 7 0.596598961004787 1102 8 0.5795461759976348 1102 9 0.5223015613577573 1102 10 0.7369843533983911 1102 11 0.7070494300672152 1102 12 0.747266943762483 1102 13 0.6808625583926684 1102 14 0.7743086303701148 1102 15 0.8285641854307499 1102 16 0.8871457485702052 1102 17 0.8909130313708786 1102 18 1.0076137760229784 1102 19 0.8760777112967588 1102 20 0.882897746414514 1102 21 0.8680550441980301 1102 22 0.9453658057228367 1102 23 0.7887361645814721 1102 24 0.7618900727538716 1102 25 0.8328785049576908 1103 1 -0.19587862621818886 1103 2 0.045755373291356705 1103 3 -0.00606150554563456 1103 4 0.08010832201749452 1103 5 -0.3357275790229508 1103 6 -0.0680206696511334 1103 7 -0.09001160716880463 1103 8 -0.03875427228243211 1103 9 0.026478851907761225 1103 10 -0.02372205999427024 1103 11 0.014692951638046736 1103 12 0.07372794521495507 1103 13 0.29755943121675443 1103 14 0.24719284344406844 1103 15 0.21040324664782872 1103 16 0.17916483346941448 1103 17 0.28092452758045283 1103 18 0.2950701166042222 1103 19 0.2244968805913743 1103 20 0.38491938010520954 1103 21 0.2706829015171266 1103 22 0.2912023250455249 1103 23 0.2669192305111565 1103 24 0.34010301566125634 1103 25 0.3075741469631552 1104 1 0.01897932231730337 1104 2 0.09898771121469707 1104 3 0.2649508244926366 1104 4 0.2178091749513129 1104 5 -0.19080245113095629 1104 6 0.12396937304364286 1104 7 0.18333919912771535 1104 8 0.26846327151816496 1104 9 0.36548278482696617 1104 10 0.2621964785752624 1104 11 0.1813633244480144 1104 12 0.41773847707256095 1104 13 0.4190393964070509 1104 14 0.31086448555803514 1104 15 0.2420910723877796 1104 16 0.4858775404525133 1104 17 0.4319143082408772 1104 18 0.4517143701188502 1104 19 0.5729719128047748 1104 20 0.533052024777021 1104 21 0.5544853324266015 1104 22 0.49330052301849037 1104 23 0.5005369752848878 1104 24 0.5278097583579466 1104 25 0.5730245575637696 1105 1 0.29582653041566687 1105 2 0.3445216508467581 1105 3 0.49275052635082517 1105 4 0.5369127850095552 1105 5 0.1746399658977964 1105 6 0.4166724099300225 1105 7 0.49633176869413376 1105 8 0.617589357771287 1105 9 0.6716362931934299 1105 10 0.6242533104140335 1105 11 0.4898918559262455 1105 12 0.7518488473230889 1105 13 0.7693889619058041 1105 14 0.7579087792623938 1105 15 0.8488943103465445 1105 16 0.9368109670033107 1105 17 1.034574183899565 1105 18 0.8282645919918252 1105 19 0.7980822714994176 1105 20 0.7815852941677903 1105 21 0.6529807562964365 1105 22 0.9533818764436504 1105 23 0.9695432270182707 1105 24 0.9691393784004703 1105 25 0.9433005120798086 1106 1 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1107 13 0.48740951353956063 1107 14 0.5607275142029451 1107 15 0.557818844899181 1107 16 0.48346866306621905 1107 17 0.6118421680385211 1107 18 0.6258474910243734 1107 19 0.6475025146105651 1107 20 0.5948208265152317 1107 21 0.6742950859602814 1107 22 0.6648977674011931 1107 23 0.7557697503304084 1107 24 0.6650192734969522 1107 25 0.5714078849528761 1108 1 0.1902498454082604 1108 2 0.28129342347501307 1108 3 0.31727758035708575 1108 4 0.28930599734230356 1108 5 -0.017801531557672945 1108 6 0.28694514687322936 1108 7 0.3026578597729497 1108 8 0.4883816377016643 1108 9 0.26962780475518855 1108 10 0.2903345533705277 1108 11 0.4353746840284316 1108 12 0.29208814543130757 1108 13 0.536793729059589 1108 14 0.5692952498696684 1108 15 0.6762805466198998 1108 16 0.661909209961344 1108 17 0.6605004581288441 1108 18 0.7418465556793166 1108 19 0.8335810867255731 1108 20 0.6286879555537226 1108 21 0.7241106620849465 1108 22 0.6913037353283755 1108 23 0.6833681579899746 1108 24 0.7313539573625292 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-1.665462970274774 2316 20 -1.5567033845699356 2316 21 -1.5192125798872382 2316 22 -1.552172256830076 2316 23 -1.429165803142908 2316 24 -1.6554989902003925 2316 25 -1.5816108913138294 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23663.0 3 1.0 4 527.0 5 3446.0 6 3912.0 7 3133.0 8 2550.0 9 2248.0 10 2166.0 11 1981.0 12 1821.0 13 1797.0 14 1821.0 15 1797.0 16 1882.0 17 1972.0 18 2060.0 19 2410.0 20 3059.0 21 3818.0 22 4988.0 23 5923.0 24 7232.0 25 8544.0 26 9633.0 27 11166.0 28 12830.0 29 15714.0 30 19493.0 31 25762.0 32 33059.0 33 43938.0 34 59967.0 35 86312.0 36 143187.0 37 317681.0 38 719296.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.25957619288586 20.603510997988465 14.074328628001883 24.062584181123793 2 15.496616464777787 21.549007823246434 40.79674647501263 22.157629236963142 3 19.443042271582897 25.71824602318981 30.663226017343636 24.175485687883654 4 13.323656507466328 18.16799958761383 38.188530886844 30.31981301807584 5 12.649055882476844 38.76493545444085 36.21090436719195 12.375104295890349 6 31.279852707777362 38.22794075109979 17.917283947512054 12.574922593610797 7 27.328559059163233 31.919998943614814 22.90966208878792 17.84177990843404 8 24.306573335823163 38.68288073155688 20.652121289856904 16.358424642763055 9 26.313985963862162 14.86501440458331 20.800193150938153 38.02080648061637 10 14.92822412959457 29.452659866439223 35.18284597252202 20.436270031444188 11 33.777100100603626 21.980382293762577 25.90995975855131 18.332557847082494 12 23.56042701332735 24.857386627634646 32.49201974990082 19.090166609137192 13 29.31858793231149 22.348160471611394 25.887509415503168 22.445742180573948 14 20.047653175411465 23.292532659399242 27.960532106169765 28.699282059019527 15 22.350742327770902 33.0073076033274 23.110295522898497 21.5316545460032 16 21.102585944056603 28.29840669133063 29.51666389013745 21.082343474475316 17 21.040498693043016 29.73242271728867 28.516368080325993 20.710710509342324 18 20.925719401168543 27.402121584361076 31.58713973362637 20.08501928084401 19 23.433413964435147 26.81922875764403 28.324649983907307 21.422707294013517 20 23.51691332654843 27.803910894418927 28.71742099979444 19.9617547792382 21 22.11011809907448 26.95377056455846 27.910154936279806 23.025956400087253 22 22.872754383737142 29.035760130654037 27.7294712107249 20.362014274883926 23 21.19078990379538 28.816439272405418 28.77023540712206 21.222535416677143 24 22.93906719908269 27.254428138924375 29.354210881001 20.452293780991944 25 22.020519377491297 28.015783362846992 28.859264176455834 21.10443308320588 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 168.0 1 168.0 2 320.5 3 473.0 4 473.0 5 473.0 6 1240.5 7 2008.0 8 2008.0 9 2008.0 10 2829.5 11 3651.0 12 3651.0 13 3651.0 14 5806.5 15 7962.0 16 7962.0 17 7962.0 18 14288.0 19 20614.0 20 20614.0 21 20614.0 22 33410.0 23 46206.0 24 46206.0 25 46206.0 26 67067.0 27 87928.0 28 87928.0 29 87928.0 30 114030.5 31 140133.0 32 140133.0 33 140133.0 34 155031.5 35 169930.0 36 169930.0 37 169930.0 38 177890.0 39 185850.0 40 185850.0 41 185850.0 42 192160.5 43 198471.0 44 198471.0 45 198471.0 46 203324.0 47 208177.0 48 208177.0 49 208177.0 50 195136.5 51 182096.0 52 182096.0 53 182096.0 54 157415.5 55 132735.0 56 132735.0 57 132735.0 58 112913.5 59 93092.0 60 93092.0 61 93092.0 62 75422.0 63 57752.0 64 57752.0 65 57752.0 66 44417.0 67 31082.0 68 31082.0 69 31082.0 70 22614.0 71 14146.0 72 14146.0 73 14146.0 74 9778.0 75 5410.0 76 5410.0 77 5410.0 78 3695.5 79 1981.0 80 1981.0 81 1981.0 82 1314.0 83 647.0 84 647.0 85 647.0 86 428.0 87 209.0 88 209.0 89 209.0 90 130.5 91 52.0 92 52.0 93 52.0 94 32.0 95 12.0 96 12.0 97 12.0 98 8.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12276926732583643 2 0.00911497376459103 3 2.514475521266491E-4 4 0.0029545087374881268 5 0.022756003467461743 6 0.030236568143229555 7 0.02891646849456465 8 0.019990080394068604 9 0.020304389834226916 10 0.02212738458714512 11 0.024453274444316622 12 0.01885856640949868 13 0.020555837386353564 14 0.008109183556084434 15 0.007354840899704486 16 0.004651779714343008 17 0.004463194050248022 18 0.005720431810881267 19 3.143094401583114E-4 20 1.2572377606332455E-4 21 3.7717132818997366E-4 22 0.0014458234247282323 23 8.172045444116095E-4 24 0.0033316800656781006 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1590789.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.65611057892555 #Duplication Level Percentage of deduplicated Percentage of total 1 84.00683883486715 58.51589655267484 2 11.141535696046162 15.521520849256753 3 2.591065165194464 5.414505649919628 4 0.909214926733533 2.5332950190624266 5 0.42925754286358514 1.495020543627188 6 0.225641720541916 0.943039478237203 7 0.15008691500775675 0.7318129523761073 8 0.09430870042203619 0.5255341812121692 9 0.06593553183286541 0.41335314267873036 >10 0.33498356996161766 4.185702644237845 >50 0.023272315803006746 1.1275512712833977 >100 0.021908943117759188 3.1266856722401233 >500 0.0033356832044967788 1.5743331091858694 >1k 0.002614454403524502 3.891748934007813 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4702 0.29557659752487603 No Hit CTGTAGGACGTGGAATATGGCAAGA 4463 0.28055260628530876 No Hit GTCCTACAGTGGACATTTCTAAATT 4442 0.27923250663664384 No Hit CTTTAGGACGTGAAATATGGCGAGG 4149 0.26081397344336676 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4138 0.26012249267501847 No Hit TATCAACGCAGAGTACTTTTTTTTT 3328 0.20920436336937204 No Hit GGTATCAACGCAGAGTACTTTTTTT 3232 0.20316962211833245 No Hit GTACATGGGAAGCAGTGGTATCAAC 2672 0.16796696482060158 No Hit GTCCTACAGTGTGCATTTCTCATTT 2614 0.1643209753147652 No Hit CTGAAGGACCTGGAATATGGCGAGA 2305 0.14489665191298154 No Hit CTGTAGGACCTGGAATATGGCGAGA 2281 0.14338796660022166 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1838 0.11554015020219525 No Hit ATTTAGAAATGTCCACTGTAGGACG 1787 0.11233419391258048 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1591 0.10001326385837468 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.286188803166228E-5 0.0 3 0.0 0.0 0.0 6.286188803166228E-5 0.0 4 0.0 0.0 0.0 6.286188803166228E-5 0.0 5 0.0 1.2572377606332455E-4 0.0 6.286188803166228E-5 0.0 6 0.0 1.2572377606332455E-4 0.0 6.286188803166228E-5 0.0 7 0.0 1.8858566409498683E-4 0.0 6.286188803166228E-5 0.0 8 0.0 1.8858566409498683E-4 0.0 6.286188803166228E-5 0.0 9 0.0 1.8858566409498683E-4 0.0 6.286188803166228E-5 0.0 10 0.0 1.8858566409498683E-4 0.0 6.286188803166228E-5 0.0 11 0.0 1.8858566409498683E-4 0.0 6.286188803166228E-5 0.0 12 0.0 1.8858566409498683E-4 0.0 6.286188803166228E-5 0.0 13 0.0 1.8858566409498683E-4 0.0 6.286188803166228E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 850 0.0 12.967882 4 TAAGACG 60 4.0836714E-4 12.66977 4 TAGAAAT 665 0.0 11.860047 4 CCACCTT 685 0.0 11.370757 13 AATGTCC 650 0.0 11.107269 8 CTGTTCG 60 0.0058774245 11.082912 9 GGACCTG 1685 0.0 10.882602 6 GTCTTAC 175 2.0008883E-11 10.86869 1 AGGACCT 1740 0.0 10.813333 5 GTTCTAA 160 5.347829E-10 10.698867 1 GTACTAG 80 3.7339184E-4 10.698867 1 ACCTTTT 740 0.0 10.652656 15 TAAGACA 270 0.0 10.558142 4 AATCCCG 135 5.7121724E-8 10.552168 19 TGTAGGA 2745 0.0 10.454291 2 TGTCCAC 710 0.0 10.302101 10 GACGTGG 1245 0.0 10.301461 7 CTGTAGG 2765 0.0 10.249587 1 GTAGGAC 2745 0.0 10.24659 3 GGCGAGG 1115 0.0 10.135759 19 >>END_MODULE