FastQCFastQC Report
Thu 26 May 2016
SRR1512545_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512545_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391657
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35460.25480416510677556No Hit
GGTATCAACGCAGAGTACTTTTTTT22950.1649113251325578No Hit
TATCAACGCAGAGTACTTTTTTTTT21370.1535579528576366No Hit
GTCCTAAAGTGTGTATTTCTCATTT18980.13638418087215456No Hit
GTCCTACAGTGGACATTTCTAAATT18030.12955778614989183No Hit
CTGTAGGACGTGGAATATGGCAAGA17970.12912664543059102No Hit
CTTTAGGACGTGAAATATGGCGAGG16380.1177014163691197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG4700.013.09231519
ACTGCCG604.2658977E-412.6041438
TAGGACC3050.012.1142054
GTCTAGA1703.6379788E-1211.7993791
GTCCTAC9750.011.7267681
AGGACGT10100.011.5375585
ACCGTGC600.006095489511.02862558
TGGCGAG10950.010.89010318
GGACGTG10450.010.8795466
GACGTGA5900.010.7596957
TAGGACG11250.010.6950134
TTAGGAC6850.010.6495273
TCCTACA11550.010.5812722
GTCCTAT1052.2577038E-510.5070671
CTTTAGG7600.010.4253731
CTGTAGG10500.010.4115481
GTCCTAA5500.010.394181
GTCTTAG1457.313247E-810.3753171
CGTGAAA7150.010.3154549
GTAGGAC10050.010.2752073
Produced by FastQC (version 0.11.5)