FastQCFastQC Report
Thu 26 May 2016
SRR1512545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512545_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391657
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46920.33715204249322933No Hit
GGTATCAACGCAGAGTACTTTTTTT30140.21657635466210426No Hit
TATCAACGCAGAGTACTTTTTTTTT30050.21592964358315303No Hit
CTGTAGGACGTGGAATATGGCAAGA20420.14673155813537386No Hit
GTCCTAAAGTGTGTATTTCTCATTT20280.145725563123672No Hit
CTTTAGGACGTGAAATATGGCGAGG19350.1390428819745095No Hit
GTCCTACAGTGGACATTTCTAAATT18470.13271948475809772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3750.011.6547444
TACTAAT951.3574345E-511.0017282
GTCCTAA6900.010.4774641
GGTATCA10550.010.459171
CTAATAC1002.3946732E-510.4512663
CTAATCT1201.5204296E-610.29291254
TTAGGAC7000.010.1798043
GTCCTAC8350.010.1390031
GACGTGA6300.010.1029457
TAAAGTG8100.010.0876415
CGAAATC1054.1092582E-59.95108113
GGACGTG10150.09.9209586
GGCGAGG5250.09.76839819
AGGACGT10350.09.7306475
CTAAAGT7750.09.6850434
GTGTAAG1901.02591E-99.5124351
GTCTTAG1901.02591E-99.5124351
GTACTAT1401.0389758E-69.5124341
GGTCTAC1701.6274498E-89.5124341
ATTGGAC800.00451043959.501153
Produced by FastQC (version 0.11.5)