FastQCFastQC Report
Thu 26 May 2016
SRR1512544_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512544_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232629
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT42110.3416275294512785No Hit
GTCCTAAAGTGTGTATTTCTCATTT41050.33302802384172364No Hit
CTGTAGGACGTGGAATATGGCAAGA36740.29806210952362794No Hit
GTATCAACGCAGAGTACTTTTTTTT33780.2740483957460031No Hit
CTTTAGGACGTGAAATATGGCGAGG32990.2676393302445424No Hit
GGTATCAACGCAGAGTACTTTTTTT20760.16842050608901785No Hit
TATCAACGCAGAGTACTTTTTTTTT19820.16079452941639374No Hit
GTCCTACAGTGTGCATTTCTCATTT19380.15722492331431437No Hit
CTGAAGGACCTGGAATATGGCGAGA17380.14099944103213538No Hit
GTCCTTCAGTGTGCATTTCTCATTT15430.12517959580701088No Hit
ATTTAGAAATGTCCACTGTAGGACG15060.12217788158480776No Hit
TTTCTAAATTTTCCACCTTTTTCAG13780.1117935729242132No Hit
GAATATGGCAAGAAAACTGAAAATC13500.10952200540470815No Hit
CTGTAGGACCTGGAATATGGCGAGA13410.1087918587020101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7000.012.8598434
GCCGGTT854.0800896E-612.25613811
TATTGCG701.1148262E-412.18549316
TTGCGAG701.1323499E-412.16514918
GGCGAGG9800.012.06860119
GTCGAGG550.003161825212.04226919
AATCCCG1501.4551915E-1111.98492519
ACCTTTT5750.011.86761215
CACCTTT6050.011.74909814
TGTAGGA22200.011.6952012
TTCCACC6200.011.60918711
GACGTGA11850.011.5947077
ATAAGGG907.672708E-611.5813793
TGCCGGT907.725112E-611.57429910
TGTCGAG907.799021E-611.56440218
ATTTAGA4100.011.521581
GTGTAGG2800.011.486561
GGACGTG22900.011.3790866
TAGAAAT4500.011.3708084
AGGACGT23150.011.3380635