FastQCFastQC Report
Thu 26 May 2016
SRR1512544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512544_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232629
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA43030.3490912513010808No Hit
GTCCTACAGTGGACATTTCTAAATT42780.3470630660158085No Hit
GTATCAACGCAGAGTACTTTTTTTT42610.34568390002182325No Hit
GTCCTAAAGTGTGTATTTCTCATTT42140.3418709116855112No Hit
CTTTAGGACGTGAAATATGGCGAGG37260.3022807349169945No Hit
GGTATCAACGCAGAGTACTTTTTTT29410.23859571695944198No Hit
TATCAACGCAGAGTACTTTTTTTTT28740.233160180394912No Hit
CTGAAGGACCTGGAATATGGCGAGA20760.16842050608901785No Hit
GTCCTACAGTGTGCATTTCTCATTT20340.16501315480976025No Hit
ATTTAGAAATGTCCACTGTAGGACG17290.14026929432943733No Hit
GTCCTTCAGTGTGCATTTCTCATTT16380.13288669989104587No Hit
TTTCTAAATTTTCCACCTTTTTCAG15760.1278568003835704No Hit
CTGTAGGACCTGGAATATGGCGAGA15560.1262342521553525No Hit
GAATATGGCAAGAAAACTGAAAATC15130.12274577346468403No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14010.11365950338666378No Hit
GGAATATGGCGAGAAAACTGAAAAT12800.10384308660594549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6750.013.3746844
GTCCTAG1700.012.3135821
GGTCTAT853.87233E-612.3135821
TCCAACG853.9612514E-612.28860518
CCTATAC752.0669476E-411.4036783
GGTATCA9700.011.1826251
GGACTGT2050.011.12328156
ACTGTGC2050.011.1214768
AGGACCT13650.011.0695055
GGCGAGG9950.010.97453119
AAAGGCG803.7582577E-410.69094855
GTCGAGG803.7865536E-410.68227519
GGACCTG13700.010.680116
AATGTCC5800.010.6460978
GTATAAA2100.010.4212351
AAATGTC6050.010.3636147
AGGACAT5400.010.2069965
ATAGGAC2053.6379788E-1210.1984123
GATATAC2800.010.1946861
GTATAGG1409.454379E-810.1946861
Produced by FastQC (version 0.11.5)