FastQCFastQC Report
Thu 26 May 2016
SRR1512543_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512543_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788766
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48790.2727578677143908No Hit
GTCCTACAGTGGACATTTCTAAATT31390.17548410468445844No Hit
CTGTAGGACGTGGAATATGGCAAGA31220.17453372883876372No Hit
GTCCTAAAGTGTGTATTTCTCATTT29840.16681891315018285No Hit
GGTATCAACGCAGAGTACTTTTTTT29750.16631577299657976No Hit
TATCAACGCAGAGTACTTTTTTTTT29630.16564491945844229No Hit
CTTTAGGACGTGAAATATGGCGAGG26540.1483704408514026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGTG400.00537249314.2125417
TCCAACG1351.8189894E-1213.32287418
GCCGGTT701.12202564E-412.177731511
AATCCCG1451.03682396E-1011.75285919
CCAACGA907.751518E-611.57148719
CTAGGAC2000.010.8959763
GGCGAGG8350.010.88488419
TAGGACT2550.010.7749164
TAGGACC5650.010.7321714
AGGACGT18800.010.6840065
CGGTTTC803.8600795E-410.66060413
GTCTTAG1706.1299943E-1010.6140861
AATGTCC4850.010.5285178
ATTAGGG909.776025E-510.5275133
GTATAAC1251.2908604E-610.4253921
ACTGTTC4350.010.217018
GGACGTG18850.010.2035896
GTCCTAA11000.010.1155451
CGTTTCC750.002696264510.1112414
GTCCTAC16950.010.0539851
Produced by FastQC (version 0.11.5)