FastQCFastQC Report
Thu 26 May 2016
SRR1512543_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512543_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788766
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63730.35627913321250515No Hit
TATCAACGCAGAGTACTTTTTTTTT42920.2399419488071665No Hit
GGTATCAACGCAGAGTACTTTTTTT42870.23966242649960923No Hit
CTGTAGGACGTGGAATATGGCAAGA33510.187335850524887No Hit
GTCCTACAGTGGACATTTCTAAATT33030.18465243637233714No Hit
CTTTAGGACGTGAAATATGGCGAGG29200.16324102761344972No Hit
GTCCTAAAGTGTGTATTTCTCATTT29200.16324102761344972No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19020.10633028579478815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5750.013.8812844
TGGACCG655.4408192E-513.1570815
ATAGGAC2500.012.9224533
CCCGTTT905.405036E-712.66694612
TCCAACG1001.4524812E-712.34371318
CGTGCGC701.0902833E-412.21523910
TAGAACG550.003063753712.0935444
CGTTTCC951.0436452E-611.99657514
TCCCGTT1052.718789E-711.76249211
ATCCCGT1001.9281124E-611.40088910
CCAACGA752.0845213E-411.39387819
AATGTCC5900.011.1110378
GGTATCA15600.011.0959921
GGCGAGG7700.010.97462319
AGGACCT12550.010.8273625
GTCCTAA8900.010.7932121
TGTAGGA19850.010.435232
TGTCCAC6750.010.41562710
TAGGACA6300.010.4070294
TAGAAAT6500.010.3791854
Produced by FastQC (version 0.11.5)