FastQCFastQC Report
Thu 26 May 2016
SRR1512542_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512542_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830712
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58540.3197662985767286No Hit
GTCCTAAAGTGTGTATTTCTCATTT53670.2931646266589174No Hit
CTGTAGGACGTGGAATATGGCAAGA52330.2858450701147969No Hit
GTATCAACGCAGAGTACTTTTTTTT46870.25602060837532065No Hit
CTTTAGGACGTGAAATATGGCGAGG44840.24493202644654102No Hit
GTCCTACAGTGTGCATTTCTCATTT31070.16971538942225756No Hit
TATCAACGCAGAGTACTTTTTTTTT28570.15605950034740582No Hit
GGTATCAACGCAGAGTACTTTTTTT28560.1560048767911064No Hit
CTGAAGGACCTGGAATATGGCGAGA25160.13743286764930804No Hit
CTGTAGGACCTGGAATATGGCGAGA21760.11886085850750965No Hit
GTCCTTCAGTGTGCATTTCTCATTT20840.11383549132796421No Hit
ATTTAGAAATGTCCACTGTAGGACG20430.11159592551968853No Hit
TTTCTAAATTTTCCACCTTTTTCAG18540.1012720733791006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACT350.002207395216.2445454
TAGGACC8750.013.1039334
CCAACGA1450.013.05705719
TCCAACG1850.011.76641418
GTGTAGG2950.011.5414111
GTCCTAA14750.011.337741
GGCGAGG12000.011.28075419
GAGGTCG600.005986832511.0556196
GTATAGA2750.010.9242241
GTTCTAA2600.010.7841691
GTCCTAG3550.010.7190741
GACGTGG15950.010.6941967
AGGACCT19250.010.682025
GTATTAA2350.010.6530541
GTCCTAC30000.010.5813251
CACCTTT8000.010.54724714
GGACCTG18550.010.5234856
GTATAAT2900.010.3591781
ACCTTTT7900.010.32073115
GTACTAA1654.305548E-910.3173221
Produced by FastQC (version 0.11.5)