FastQCFastQC Report
Thu 26 May 2016
SRR1512542_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512542_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830712
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA60780.3320019751877958No Hit
GTCCTACAGTGGACATTTCTAAATT60170.32866993825353197No Hit
GTATCAACGCAGAGTACTTTTTTTT56020.30600116238927805No Hit
GTCCTAAAGTGTGTATTTCTCATTT56000.30589191527667925No Hit
CTTTAGGACGTGAAATATGGCGAGG51860.28327776296872476No Hit
TATCAACGCAGAGTACTTTTTTTTT38100.2081157495007407No Hit
GGTATCAACGCAGAGTACTTTTTTT35480.19380437775029608No Hit
GTCCTACAGTGTGCATTTCTCATTT32940.17992999445024668No Hit
CTGAAGGACCTGGAATATGGCGAGA29910.16337905689152638No Hit
CTGTAGGACCTGGAATATGGCGAGA25100.1371051263115116No Hit
ATTTAGAAATGTCCACTGTAGGACG23510.12841998085990589No Hit
TTTCTAAATTTTCCACCTTTTTCAG21570.11782301093782091No Hit
GTCCTTCAGTGTGCATTTCTCATTT20310.11094044284409564No Hit
GAATATGGCAAGAAAACTGAAAATC19660.10738991168463417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT1058.913048E-1114.47546211
CCAGTCG400.00528038214.2496729
TACGGCT500.001497516113.3026014
CCAACGT655.4715005E-513.14923219
TAGGACC9450.012.7696934
TCCAACG1600.012.46471718
ATTAGAC1103.8045982E-812.0932733
ACGAAAT1950.011.69076112
TAGAACG752.0704337E-411.402234
CCGGTTT1354.7384674E-911.2577712
CCACCTT6250.011.24589813
AGGACGT31200.011.2377755
GGACGTG30850.011.1176536
TGGACGG600.005867786211.0855025
ATGCGTG600.00587495211.0836847
CGGTTTC1553.1468517E-1011.03019613
TGTCGAG1752.0008883E-1110.85363118
GGCGAGG14300.010.82487319
TTAGACT1502.1263986E-910.7687734
GGTATCA13350.010.6896841
Produced by FastQC (version 0.11.5)