FastQCFastQC Report
Thu 26 May 2016
SRR1512541_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512541_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817752
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT43710.24046184518020058No Hit
GTATCAACGCAGAGTACTTTTTTTT43100.23710605187066222No Hit
GTCCTAAAGTGTGTATTTCTCATTT39980.21994199428744957No Hit
CTGTAGGACGTGGAATATGGCAAGA37680.20728900312033766No Hit
CTTTAGGACGTGAAATATGGCGAGG34420.18935476346608338No Hit
GGTATCAACGCAGAGTACTTTTTTT27100.14908524375162288No Hit
TATCAACGCAGAGTACTTTTTTTTT26600.1463345934979029No Hit
GTCCTACAGTGTGCATTTCTCATTT21450.11800289588458712No Hit
CTGAAGGACCTGGAATATGGCGAGA18320.10078382529630005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGTA604.257869E-412.6073519
TAGGACC6450.012.483834
GTCTTAC1402.6739144E-1012.1999531
TGTCGAG1052.853376E-711.71878818
CGTTCAG658.243035E-411.65491810
GGCGAGG11350.011.00978219
GTCCTAA12300.010.6188021
AGGACGT24100.010.6129315
GTATAAA3050.010.5411371
TTAGGAC14400.010.5261643
GGACGTG23700.010.5122596
GTCTAAG1551.704575E-810.3711181
CCACCTT6950.010.36242713
GGTATCA11750.010.34629251
TGTAGGA24750.010.3350652
GTCCTAC21350.010.2587851
GTCGAGG1201.5938458E-610.2556419
ATACTGT3150.010.2248516
GTAGGAC24050.010.12353
CTGTAGG23150.010.112121
Produced by FastQC (version 0.11.5)