FastQCFastQC Report
Thu 26 May 2016
SRR1512541_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512541_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817752
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54210.2982255005083202No Hit
GTCCTACAGTGGACATTTCTAAATT45540.25052922510881576No Hit
CTGTAGGACGTGGAATATGGCAAGA44730.24607317169778936No Hit
GTCCTAAAGTGTGTATTTCTCATTT39800.21895176019611035No Hit
CTTTAGGACGTGAAATATGGCGAGG39000.2145507197901584No Hit
TATCAACGCAGAGTACTTTTTTTTT37510.20635378203407284No Hit
GGTATCAACGCAGAGTACTTTTTTT36600.20134759857230247No Hit
GTCCTACAGTGTGCATTTCTCATTT23630.12999573099080625No Hit
CTGAAGGACCTGGAATATGGCGAGA21370.11756279184399192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG250.006037509518.997258
TGTCCGA400.005287528514.2467610
TAGGACC7050.013.3462054
CCTTGCG550.003072120712.0891589
GTGCGCC951.3638675E-510.99749911
GGACCTG13850.010.9066586
AATCCCG1408.407369E-910.85258419
AGGACCT14900.010.8436225
AGGACGT24200.010.8394055
GGACGTG23950.010.8293286
TGTAGGA25100.010.6779312
GGCGAGG11300.010.50443719
ACCTTTT5450.010.45518715
GACGTGG11950.010.4138647
GTAGGAC24300.010.4036833
ATAAGAC1752.3464963E-1010.3187543
CTAATAC1201.5153128E-610.2961253
TTAGGAC14500.010.2906643
CTGTAGG25450.010.2455861
GATATAC4300.010.1803661
Produced by FastQC (version 0.11.5)