FastQCFastQC Report
Thu 26 May 2016
SRR1512540_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512540_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399570
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC33850.24185999985709897No Hit
GTATCAACGCAGAGTACTTTTTTTT32420.23164257593403687No Hit
CCCATGTACTCTGCGTTGATACCAC27120.19377380195345711No Hit
GGTATCAACGCAGAGTACTTTTTTT20530.14668791128703815No Hit
TATCAACGCAGAGTACTTTTTTTTT19270.1376851461520324No Hit
GTCCTACAGTGGACATTTCTAAATT18250.13039719342369444No Hit
GTCCTAAAGTGTGTATTTCTCATTT17750.12682466757646993No Hit
CTGTAGGACGTGGAATATGGCAAGA15850.1132490693570168No Hit
GAGTACATGGGAAGCAGTGGTATCA14680.10488935887451145No Hit
CTTTAGGACGTGAAATATGGCGAGG14240.10174553612895389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAT806.609298E-815.0480691
AGGACCG456.902717E-414.7375975
AAACTCG400.005374941514.2112556
GTAATAC1251.4715624E-912.1269373
ACCGTCC550.003185643312.0303938
ACGAAAT951.0784479E-611.96350612
GTACTAA957.4227737E-611.6160531
TAGGACC2400.011.0531984
CTAGCAC1303.3971446E-810.9317343
CGAAATC1053.573472E-610.82917813
GGCGAGG4750.010.76483819
GTATAAT1602.5138434E-910.6590491
GTATTAC1457.285962E-810.3779781
GTATTAG1551.7429556E-810.355661
CTCCTAG1551.7429556E-810.355661
TGGCGAG9200.010.29062218
TCTAGGT750.002707736810.1057813
GGCGTGC750.002759767510.0826158
AGGACTG3200.010.0663065
TCGAACT1606.502887E-910.06088819
Produced by FastQC (version 0.11.5)