FastQCFastQC Report
Thu 26 May 2016
SRR1512540_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512540_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399570
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41570.2970197989382453No Hit
GTACATGGGAAGCAGTGGTATCAAC40250.28758833070157264No Hit
TATCAACGCAGAGTACTTTTTTTTT27200.19434540608901305No Hit
GGTATCAACGCAGAGTACTTTTTTT26720.19091578127567754No Hit
CCCATGTACTCTGCGTTGATACCAC26430.18884371628428734No Hit
GTCCTACAGTGGACATTTCTAAATT18860.13475567495730834No Hit
GTCCTAAAGTGTGTATTTCTCATTT18350.13111169859313931No Hit
GAGTACATGGGAAGCAGTGGTATCA18160.12975413877119402No Hit
CTGTAGGACGTGGAATATGGCAAGA17920.12803932636452625No Hit
CTTTAGGACGTGAAATATGGCGAGG16410.11725029830590825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3550.013.3831514
CGTTTCC802.0046173E-613.05923314
TAGACCG550.00306325912.0935035
ATCCCGT802.867979E-511.87415110
TCCAACG1052.730285E-711.75812218
TCTATAC1451.2114469E-911.1403193
CTAATAC1158.787938E-710.7414343
GTCCTAA5850.010.7336771
ATAGTAC1605.4205884E-1010.6897933
GCCGGTT803.7726588E-410.68673611
TCCCGTT909.519493E-510.55480211
TCTAGGA2151.8189894E-1210.164972
GTAGGAC10200.010.1541383
CAAGTCG856.589901E-410.0591836
GTCCTAC10200.09.980311
TGTAGGA10950.09.8925312
GTCTAGG1356.309965E-79.8663091
GTATTAA1553.9679435E-89.8208421
TAGTACA1554.0456143E-89.8085564
TCCTACA11550.09.7899782
Produced by FastQC (version 0.11.5)