FastQCFastQC Report
Thu 26 May 2016
SRR1512539_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512539_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2745181
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60990.22217114281353395No Hit
GTCCTACAGTGGACATTTCTAAATT60570.22064118905092234No Hit
GTCCTAAAGTGTGTATTTCTCATTT55260.20129820219504652No Hit
CTGTAGGACGTGGAATATGGCAAGA55080.20064250772535583No Hit
CTTTAGGACGTGAAATATGGCGAGG46640.1698977225909694No Hit
GTACATGGGAAGCAGTGGTATCAAC39440.14366994380334122No Hit
GTCCTACAGTGTGCATTTCTCATTT37730.1374408463412795No Hit
GGTATCAACGCAGAGTACTTTTTTT36710.1337252443463655No Hit
TATCAACGCAGAGTACTTTTTTTTT35430.1290625281174538No Hit
CCCATGTACTCTGCGTTGATACCAC29210.10640464144258611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG4800.012.846241
GCCGGTT1850.012.80571111
TAGGACC11450.012.5795634
ATACCGC550.003128034812.060436
TCCAACG2300.011.93888518
CCGGTTT2150.011.4606712
ATTCCGT752.1241177E-411.37126356
CGGTAGG700.001017319511.3662581
CGGTTTC2050.011.10021513
GGCGAGG16050.011.09155319
GTCCTAA16500.010.7290591
GTACCGT803.8616516E-410.6605596
TACACCG803.8616516E-410.6605595
TGTAGGA35350.010.5885162
AGTGCCG909.766914E-510.5289475
CGTGCGC909.768726E-510.52875510
GGACCTG20950.010.4937676
TAGGACA10600.010.4594154
CCAACGA1002.487994E-510.41602919
GTCCTAC30850.010.3806921
Produced by FastQC (version 0.11.5)