FastQCFastQC Report
Thu 26 May 2016
SRR1512539_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512539_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2745181
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69240.2522238060076913No Hit
CTGTAGGACGTGGAATATGGCAAGA67650.24643183819209008No Hit
GTCCTACAGTGGACATTTCTAAATT67130.24453760972409472No Hit
GTCCTAAAGTGTGTATTTCTCATTT59390.21634274752739438No Hit
CTTTAGGACGTGAAATATGGCGAGG57000.20763658206872332No Hit
TATCAACGCAGAGTACTTTTTTTTT46540.1695334478855857No Hit
GTACATGGGAAGCAGTGGTATCAAC45190.16461573936290538No Hit
GGTATCAACGCAGAGTACTTTTTTT44930.1636686251289077No Hit
GTCCTACAGTGTGCATTTCTCATTT40120.14614701179995052No Hit
CCCATGTACTCTGCGTTGATACCAC33180.12086634724632No Hit
CTGAAGGACCTGGAATATGGCGAGA30090.10961025884996291No Hit
CTGTAGGACCTGGAATATGGCGAGA29370.10698748097120007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATCG602.5716981E-514.2488119
TAGGACC12150.013.2954324
GGCGAGG14700.012.27386119
TACCCGT550.003071813612.089686
TGTAGGA37300.011.3872962
GTAGGAC36350.011.1359453
CTGTAGG38150.011.0472411
TCACGAC700.00148921810.8596013
AGGACGT35250.010.7831725
TGGCGAG36900.010.757483518
TTAGGAC22350.010.756353
AGGACCT23350.010.7439185
GGGCCGA803.7762016E-410.6864156
GGACGTG34550.010.6675126
GACGTGG17550.010.5546757
GGACCGT909.5300646E-510.5544826
GGACCTG22050.010.5114036
TAGGACA11100.010.4439964
GACGTGA18450.010.4002177
TAGGACG37850.010.34334854
Produced by FastQC (version 0.11.5)