FastQCFastQC Report
Thu 26 May 2016
SRR1512538_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512538_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172111
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT57910.1825598158450319No Hit
GTATCAACGCAGAGTACTTTTTTTT55550.17511997530981735No Hit
CTGTAGGACGTGGAATATGGCAAGA50870.1603663932315105No Hit
GTCCTAAAGTGTGTATTTCTCATTT49820.15705629468830062No Hit
GTACATGGGAAGCAGTGGTATCAAC42310.13338120891734243No Hit
CTTTAGGACGTGAAATATGGCGAGG41840.13189954575990562No Hit
GTCCTACAGTGTGCATTTCTCATTT34700.10939087566607852No Hit
GGTATCAACGCAGAGTACTTTTTTT32440.10226628261116966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11500.013.1033364
TCCAACG3550.012.2698918
TATTCCG701.1162838E-412.1850445
GTGTAGG5500.012.0781631
TTACGGC550.003125245212.0619623
CCAACGT1950.011.65655819
GGCGAGG14200.011.60525219
TGTAGGA35050.010.8968172
GGACGTG31800.010.7885746
CCGGTTT2900.010.78685712
AGGACGT32700.010.7524835
GCCGGTT3000.010.74191211
GTAGGAC33050.010.6099393
GACGTGA18000.010.4776337
TTAGGAC21450.010.3830083
GTCCTAC32100.010.2237851
TAGGACG35350.010.2145244
AGGACCT21250.010.2131375
GACCGTG1401.0045369E-710.1542037
ACGTGAA20450.010.1234148
Produced by FastQC (version 0.11.5)