FastQCFastQC Report
Thu 26 May 2016
SRR1512538_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512538_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172111
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70850.2233528397965897No Hit
CTGTAGGACGTGGAATATGGCAAGA61870.1950436160651377No Hit
GTCCTACAGTGGACATTTCTAAATT61480.19381415089194545No Hit
GTCCTAAAGTGTGTATTTCTCATTT53540.16878350095567274No Hit
CTTTAGGACGTGAAATATGGCGAGG50090.15790746288512603No Hit
TATCAACGCAGAGTACTTTTTTTTT49240.15522785930252755No Hit
GTACATGGGAAGCAGTGGTATCAAC49240.15522785930252755No Hit
GGTATCAACGCAGAGTACTTTTTTT46250.1458019596413871No Hit
GTCCTACAGTGTGCATTTCTCATTT39190.12354548753180453No Hit
CCCATGTACTCTGCGTTGATACCAC35620.112291152484891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12900.012.4490734
CGTGCGC1201.00444595E-811.8736910
GGCGAGG14150.011.67792719
AAGGCGT1501.7644197E-1011.3989216
GTACCGT1104.979902E-711.2262116
GGCGTGC1201.2787677E-711.082118
GTCCTAC33150.010.9923331
TGTAGGA35650.010.8751142
GATATAC9300.010.7418981
TTGGCGA803.782362E-410.68463617
AGGACGT33600.010.6338035
GGACGTG32950.010.5513536
CTGTAGG36500.010.478711
GACGTGA17850.010.4302227
TCCTACA36900.010.3522052
TGGCGAG32850.010.35001918
GTAGGAC35950.010.2821493
TTAGGAC21450.010.2776513
GCGCCAC1856.002665E-1110.26848913
TATACAC14050.010.2125423
Produced by FastQC (version 0.11.5)