FastQCFastQC Report
Thu 26 May 2016
SRR1512537_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512537_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411220
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66280.2748815952090643No Hit
GTCCTACAGTGGACATTTCTAAATT55670.23087897412927896No Hit
GTCCTAAAGTGTGTATTTCTCATTT55430.22988362737535356No Hit
CTGTAGGACGTGGAATATGGCAAGA51120.21200885858610993No Hit
CTTTAGGACGTGAAATATGGCGAGG49160.2038801934290525No Hit
GGTATCAACGCAGAGTACTTTTTTT41360.17153142392647705No Hit
TATCAACGCAGAGTACTTTTTTTTT39130.16228299367125357No Hit
GTCCTACAGTGTGCATTTCTCATTT32260.13379119284013902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT500.001525833313.26984811
TAGGACC11300.012.4140124
AGTGCGG701.114744E-412.18659510
CCATCCG658.194784E-411.6633699
CCAACGA1407.185008E-1011.49853919
GCCCTAT958.489862E-611.4775921
GTATTAG2500.011.1019621
CTATACG600.00597828211.0579764
TCCAACG2250.010.94103418
GGACCTG21050.010.7615456
AGGACCT22250.010.6071375
CACCTTT8700.010.57764114
GAACAGT6100.010.5659356
GGCGAGG14750.010.52867219
ACCTTTT9400.010.294579515
GATATAC5900.010.2484821
GTCTAGA3100.010.2322231
AATGTCC9250.010.2220638
TAGGTCG750.00269926510.1101495
TGGCGAG34500.010.01708318
Produced by FastQC (version 0.11.5)