FastQCFastQC Report
Thu 26 May 2016
SRR1512537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512537_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411220
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82320.3414039365964118No Hit
GTCCTACAGTGGACATTTCTAAATT60810.2521959837758479No Hit
CTGTAGGACGTGGAATATGGCAAGA60560.251159164240509No Hit
GTCCTAAAGTGTGTATTTCTCATTT59660.2474266139132887No Hit
CTTTAGGACGTGAAATATGGCGAGG57500.23846849312796015No Hit
TATCAACGCAGAGTACTTTTTTTTT55780.2313351747248281No Hit
GGTATCAACGCAGAGTACTTTTTTT53990.22391154685180115No Hit
GTCCTACAGTGTGCATTTCTCATTT34300.14225164024850492No Hit
CTGAAGGACCTGGAATATGGCGAGA30400.12607725549721718No Hit
GTACATGGGAAGCAGTGGTATCAAC26880.11147883643964467No Hit
ATTTAGAAATGTCCACTGTAGGACG26280.10899046955483117No Hit
CTGTAGGACCTGGAATATGGCGAGA26250.1088660512105905No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25890.10737303107970239No Hit
TTTCTAAATTTTCCACCTTTTTCAG25450.10554822869750582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1800.012.66253319
TCCAACG2800.012.21055218
TAGGACC11950.012.0872584
TAAGACG802.8592249E-511.8785144
TACACCG658.003551E-411.6962545
GGCGAGG16750.011.45297719
CCACCTT8850.011.2696113
CACCTTT9500.011.09729814
TTAGGAC22300.010.6527143
TGTAGGA36350.010.639352
GGACCTG22650.010.6106576
AGGACGT33500.010.6095495
TGGCGAG37900.010.57460418
GGACGTG33250.010.5706096
TAGGACA11000.010.4530934
ACCTTTT10000.010.44745615
AGGACCT24350.010.4203535
CTGTAGG36600.010.3452511
GTAGGAC36050.010.2534353
GACGTGA19850.010.1450987
Produced by FastQC (version 0.11.5)