FastQCFastQC Report
Thu 26 May 2016
SRR1512536_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512536_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314261
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT69100.20849293402058558No Hit
CTGTAGGACGTGGAATATGGCAAGA65010.19615232475655964No Hit
GTCCTAAAGTGTGTATTTCTCATTT59660.1800099630053276No Hit
GTATCAACGCAGAGTACTTTTTTTT57040.172104731643042No Hit
CTTTAGGACGTGAAATATGGCGAGG52720.15907015168690697No Hit
GTCCTACAGTGTGCATTTCTCATTT35250.10635855172540726No Hit
GGTATCAACGCAGAGTACTTTTTTT34250.1033412878466723No Hit
TATCAACGCAGAGTACTTTTTTTTT33510.10110851257640843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTG350.002208985916.2433579
CGTATAC604.178703E-412.636023
TAGGACC12050.012.1903514
TCGCAAC550.003099736512.07519816
GGCCGAT550.00312554112.0618377
TCCAACG3050.011.79991618
GTCCTAG4600.011.4157621
GGCGAGG16650.011.264519
CAACCGT700.001522627810.83087356
GACGTGA20150.010.67657
AGGACGT39100.010.6161965
GGACGTG39200.010.4924096
TGGCGAG37150.010.452518
GTAGGAC38600.010.4345373
GTCCTAC35600.010.3533241
TTAGGAC22400.010.2808693
TCCCGTT3050.010.25537411
AATGTCC8800.010.2085868
TGTAGGA40550.010.189992
TAGGACG41450.010.1286324
Produced by FastQC (version 0.11.5)