FastQCFastQC Report
Thu 26 May 2016
SRR1512536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512536_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314261
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79380.23951040669398094No Hit
CTGTAGGACGTGGAATATGGCAAGA79110.2386957454467225No Hit
GTCCTACAGTGGACATTTCTAAATT73080.2205016442579507No Hit
GTCCTAAAGTGTGTATTTCTCATTT63810.19253160810207764No Hit
CTTTAGGACGTGAAATATGGCGAGG61290.18492810312766556No Hit
TATCAACGCAGAGTACTTTTTTTTT51820.15635461419604552No Hit
GGTATCAACGCAGAGTACTTTTTTT50260.151647682545219No Hit
GTCCTACAGTGTGCATTTCTCATTT38810.11710001113370372No Hit
GTACATGGGAAGCAGTGGTATCAAC36730.11082410226593499No Hit
CTGAAGGACCTGGAATATGGCGAGA34860.10518181881270063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG15250.012.33051919
TAGGACC11750.012.211094
CCAACGA2300.011.14864119
GACGTGG20850.010.9799877
AGGACGT40500.010.7924035
GGACGTG39650.010.7573956
AGGACCT24450.010.6484675
GGACCTG22850.010.5596976
TGTAGGA40650.010.5417252
GTAGGAC39100.010.4493053
CTAGTAC2053.6379788E-1210.1968153
TTAGACG750.002644284410.1354754
TCCAACG3300.010.073173518
CGTGGAA22500.010.0481319
CTGTAGG41800.010.0343891
CCACCTT10700.010.03149913
TAGGACG43800.010.0226654
ACGTGGA22950.09.9337418
TGGCGAG38050.09.90941518
TTAGGAC23400.09.9076283
Produced by FastQC (version 0.11.5)