##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512536_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3314261 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.39829090104853 33.0 31.0 34.0 29.0 34.0 2 31.69303564203302 33.0 31.0 34.0 30.0 34.0 3 31.860646159128688 34.0 31.0 34.0 30.0 34.0 4 35.347898370104225 37.0 35.0 37.0 33.0 37.0 5 34.78867113965979 37.0 35.0 37.0 32.0 37.0 6 35.03159558043256 37.0 35.0 37.0 32.0 37.0 7 34.99046514441681 37.0 35.0 37.0 32.0 37.0 8 34.96733117880577 37.0 35.0 37.0 32.0 37.0 9 36.53619404144695 39.0 37.0 39.0 32.0 39.0 10 36.48303226571474 39.0 37.0 39.0 32.0 39.0 11 36.51965551294844 39.0 37.0 39.0 32.0 39.0 12 36.439842547101755 39.0 37.0 39.0 32.0 39.0 13 36.401851875878215 39.0 37.0 39.0 32.0 39.0 14 37.66674441149927 40.0 38.0 41.0 32.0 41.0 15 37.66236937887511 40.0 38.0 41.0 32.0 41.0 16 37.64100262471785 40.0 38.0 41.0 32.0 41.0 17 37.611623224604216 40.0 38.0 41.0 32.0 41.0 18 37.555346727369994 40.0 38.0 41.0 32.0 41.0 19 37.58909814284391 40.0 38.0 41.0 32.0 41.0 20 37.56587667658039 40.0 38.0 41.0 32.0 41.0 21 37.51422292933478 40.0 38.0 41.0 32.0 41.0 22 37.44466021233693 40.0 38.0 41.0 32.0 41.0 23 37.33819424601744 40.0 38.0 41.0 32.0 41.0 24 37.304573176343084 40.0 38.0 41.0 32.0 41.0 25 37.242101029460265 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2734172345115766 1101 2 0.3272138110140581 1101 3 0.3365086275542204 1101 4 0.34423416709014987 1101 5 0.13113157363807204 1101 6 0.2764246593995523 1101 7 0.3805324643815524 1101 8 0.43641224724772343 1101 9 0.5251020330743827 1101 10 0.5812735996264138 1101 11 0.5208367395715641 1101 12 0.5231232321170936 1101 13 0.4656607348323192 1101 14 0.638067380058466 1101 15 0.6120103642914501 1101 16 0.6000535736813504 1101 17 0.534017008795999 1101 18 0.6047049900892532 1101 19 0.6347788939063861 1101 20 0.6303117802269469 1101 21 0.6317655778278137 1101 22 0.6610017594060622 1101 23 0.7609244576507095 1101 24 0.7122696418876799 1101 25 0.6753753487942475 1102 1 0.20653230533244837 1102 2 0.40132522343554555 1102 3 0.39357942514736166 1102 4 0.3847280316091073 1102 5 0.06346321161559132 1102 6 0.2852893141405346 1102 7 0.4205257139945644 1102 8 0.4692670790117859 1102 9 0.5207929584593316 1102 10 0.5004523025434366 1102 11 0.4126087937453562 1102 12 0.4878823325310364 1102 13 0.55365148602624 1102 14 0.6509862241128843 1102 15 0.6110487833042626 1102 16 0.6607356731292882 1102 17 0.7261561443585123 1102 18 0.7229331298920982 1102 19 0.7615621017368355 1102 20 0.6682291694565876 1102 21 0.690997119980878 1102 22 0.7187677692678349 1102 23 0.6598416024697897 1102 24 0.6923438185538373 1102 25 0.682113554044804 1103 1 -0.0479317974772826 1103 2 -0.0018965705765516816 1103 3 0.10091316622187918 1103 4 0.20978510309399923 1103 5 -0.14447300390755657 1103 6 0.11131730090614411 1103 7 0.15609263390290096 1103 8 0.2735797526395274 1103 9 0.3966916224928738 1103 10 0.27846198516849796 1103 11 0.3419251886255026 1103 12 0.34334446786672856 1103 13 0.39414873377322834 1103 14 0.4785227550213875 1103 15 0.5221922025825592 1103 16 0.5050480520043692 1103 17 0.5078657098193915 1103 18 0.5970534037173252 1103 19 0.5352378885422269 1103 20 0.4970751390823196 1103 21 0.4805378216395937 1103 22 0.49909848175871474 1103 23 0.48122428722444255 1103 24 0.49252700733555344 1103 25 0.6293267412790655 1104 1 -0.13511153028398937 1104 2 -0.003058338208290934 1104 3 0.017962986791193458 1104 4 0.0485636480474696 1104 5 -0.3431121308501588 1104 6 -0.02840931599644847 1104 7 0.054498316734289176 1104 8 0.1161163940802794 1104 9 0.1465463831168634 1104 10 0.2166166861050769 1104 11 0.16466358826590266 1104 12 0.230348085955697 1104 13 0.2328295682180226 1104 14 0.19728759397590068 1104 15 0.1628296052220719 1104 16 0.18484526217038422 1104 17 0.38725203476946746 1104 18 0.3826591572893534 1104 19 0.27471278666833854 1104 20 0.29535245712781943 1104 21 0.2909971199808723 1104 22 0.3773979062541386 1104 23 0.3538142052095168 1104 24 0.3189191610195934 1104 25 0.2949902663735742 1105 1 0.1618229384312997 1105 2 0.2556458454274022 1105 3 0.2700473999714994 1105 4 0.3339653511204048 1105 5 0.1269952367914584 1105 6 0.2892026795607521 1105 7 0.35966677286165094 1105 8 0.46306567212545247 1105 9 0.6811409777114577 1105 10 0.49937122146235424 1105 11 0.47682945276054056 1105 12 0.4235357942119009 1105 13 0.5808710343416834 1105 14 0.6745515702809755 1105 15 0.7046400457996356 1105 16 0.6681588497305455 1105 17 0.6135719162948305 1105 18 0.6915299458861739 1105 19 0.6379227089193549 1105 20 0.57296075035255 1105 21 0.5679908260156736 1105 22 0.6303634745621665 1105 23 0.6080015432324686 1105 24 0.5822327485797558 1105 25 0.6810361752961924 1106 1 0.27848461438480854 1106 2 0.3141751800161323 1106 3 0.30724097275615847 1106 4 0.24879224540475064 1106 5 0.018630904440826157 1106 6 0.25269674472814785 1106 7 0.3496082734713397 1106 8 0.36786154166718177 1106 9 0.5359124596445639 1106 10 0.4838789159072263 1106 11 0.4183813072992564 1106 12 0.4931145677312756 1106 13 0.5416273589129688 1106 14 0.5489623316991583 1106 15 0.5852088327476324 1106 16 0.6181603932500792 1106 17 0.6010569055673685 1106 18 0.6743687870117085 1106 19 0.6800711337576715 1106 20 0.6102450820857683 1106 21 0.5867381055624961 1106 22 0.6122240330075357 1106 23 0.6728034325003804 1106 24 0.740366787816022 1106 25 0.7426616557166454 1107 1 0.10131478713752884 1107 2 0.19210318159179351 1107 3 0.13697653949717292 1107 4 0.1715653079347419 1107 5 -0.11798982816697645 1107 6 0.15272123140154292 1107 7 0.18841546829477096 1107 8 0.21619519312447721 1107 9 0.3434571687360375 1107 10 0.24103818802944943 1107 11 0.2891452839045954 1107 12 0.25140494165494687 1107 13 0.3284839468547247 1107 14 0.37791105493207056 1107 15 0.3426078005050712 1107 16 0.2940720465492035 1107 17 0.3474399121202154 1107 18 0.2840509089264458 1107 19 0.3400233315126542 1107 20 0.35016375895369123 1107 21 0.31138090051094736 1107 22 0.4004717985985877 1107 23 0.36644685938750854 1107 24 0.284364320897744 1107 25 0.2310426531455505 1108 1 -0.03615148269032886 1108 2 0.1290489947696365 1108 3 0.12179783060517835 1108 4 0.17848708097208288 1108 5 -0.1264810821670963 1108 6 0.04373340317776808 1108 7 0.15744912374044873 1108 8 0.26588468403075893 1108 9 0.38368118289125874 1108 10 0.17701180620953494 1108 11 0.2562742782967362 1108 12 0.1054827157527285 1108 13 0.29491441782668915 1108 14 0.3359949933995239 1108 15 0.31795280017838223 1108 16 0.38619287360213406 1108 17 0.40147713115435835 1108 18 0.40586570296304814 1108 19 0.3556085673666374 1108 20 0.4076724163332486 1108 21 0.4679899113954704 1108 22 0.37443597345700397 1108 23 0.47362645748682297 1108 24 0.4906897842956752 1108 25 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-1.3639358461771138 2314 18 -1.217246344333475 2314 19 -1.1048025820388858 2314 20 -1.1447487288268121 2314 21 -1.1550770176305676 2314 22 -1.050409258903585 2314 23 -0.9535634284784322 2314 24 -1.106902157087383 2314 25 -1.2465697564514215 2315 1 -0.8029280301538648 2315 2 -0.7256104812175259 2315 3 -0.6763421279724326 2315 4 -0.47435034429373957 2315 5 -0.46704454688563146 2315 6 -0.6932983040283105 2315 7 -0.8320662777314993 2315 8 -1.0806955057808025 2315 9 -1.0765377520916246 2315 10 -0.9658837448288864 2315 11 -0.9249119333331777 2315 12 -0.8621158085381495 2315 13 -0.912980300338667 2315 14 -1.0140851419308703 2315 15 -1.2117100052136323 2315 16 -1.080524141180419 2315 17 -1.2059106825141939 2315 18 -1.1192839323888464 2315 19 -1.1337978717649548 2315 20 -1.1153274045677293 2315 21 -0.8714785026764531 2315 22 -1.0259578356451513 2315 23 -0.9669815523723884 2315 24 -1.08198934916566 2315 25 -0.9997935737718961 2316 1 -0.6837659643466445 2316 2 -0.6767394990549356 2316 3 -0.7759808285264214 2316 4 -0.7334076752512786 2316 5 -0.5152297902113006 2316 6 -0.7770659644761864 2316 7 -1.3196232670660137 2316 8 -1.07227735388215 2316 9 -1.1129686595414228 2316 10 -1.2505759866348356 2316 11 -1.280926830689502 2316 12 -1.1915432722816277 2316 13 -1.1045312156856255 2316 14 -1.3192329416669892 2316 15 -1.2528161126488868 2316 16 -1.3879735062446485 2316 17 -1.4232716133596526 2316 18 -1.535792473075226 2316 19 -1.4721624862893208 2316 20 -1.6350869201288916 2316 21 -1.5611228768205194 2316 22 -1.4937023514794063 2316 23 -1.52111847035993 2316 24 -1.6021528628961121 2316 25 -1.4956187598365176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32864.0 3 3.0 4 755.0 5 5501.0 6 6539.0 7 5151.0 8 4189.0 9 3774.0 10 3357.0 11 3383.0 12 3129.0 13 2995.0 14 3053.0 15 3112.0 16 3169.0 17 3311.0 18 3676.0 19 4144.0 20 5343.0 21 7012.0 22 8978.0 23 11340.0 24 13740.0 25 16267.0 26 18981.0 27 21544.0 28 25048.0 29 30535.0 30 39195.0 31 51367.0 32 66341.0 33 87321.0 34 118910.0 35 163631.0 36 244939.0 37 461760.0 38 1829904.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.4273759893662 19.981028336656394 13.60310555253459 24.988490121442812 2 14.455877658242326 21.06989897185563 41.50098698721775 22.973236382684302 3 18.543293936395834 25.22251862204557 30.470636050687084 25.76355139087151 4 12.205934446985072 17.735148343070392 39.0362573480557 31.02265986188884 5 12.521461377971391 38.477896289928424 36.466141129538585 12.534501202561602 6 30.980676555943464 38.33157084015394 18.321780552698545 12.365972051204048 7 27.320943300283524 31.899199833418084 22.89647326164074 17.883383604657656 8 24.210024119857557 38.31161600631528 20.912245422040172 16.56611445178699 9 26.03509405117598 15.194969130428355 20.790058244645618 37.97987857375005 10 14.243912243475856 29.88205796937871 35.36365685783068 20.510372929314755 11 33.66736956727097 22.523995650406395 25.431571615129172 18.377063167193462 12 22.73041699965956 25.166415966274965 32.77965970741695 19.323507326648524 13 28.664904953821434 22.385694392719113 26.197018085724753 22.7523825677347 14 19.922586143058417 23.012743080084146 28.408236032900437 28.656434743957004 15 22.251259684337306 32.19335811308536 23.630854322036786 21.924527880540552 16 21.003180960468395 28.83571897824206 29.025562658221926 21.135537403067616 17 20.878801211148993 29.406242474828588 28.70500965937792 21.009946654644498 18 20.60108707558081 28.077032518423948 31.07872622719958 20.24315417879566 19 22.862424031394625 27.37630642466141 28.31282258935646 21.448446954587506 20 22.826188953897987 28.10948643684806 28.9549902436066 20.109334365647356 21 22.045889170568508 27.32597399227456 27.9829258298871 22.64521100726983 22 22.215017464413393 29.009724310897937 28.097821232961035 20.67743699172764 23 21.329891973734792 28.623550703428947 28.56220848870042 21.48434883413584 24 22.21158453351807 27.63015763626467 29.333367931804 20.824889898413257 25 21.861504319975378 28.068148045337487 28.809279913585407 21.261067721101725 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 190.0 1 190.0 2 485.5 3 781.0 4 781.0 5 781.0 6 2204.5 7 3628.0 8 3628.0 9 3628.0 10 5556.5 11 7485.0 12 7485.0 13 7485.0 14 12335.0 15 17185.0 16 17185.0 17 17185.0 18 30697.5 19 44210.0 20 44210.0 21 44210.0 22 70308.5 23 96407.0 24 96407.0 25 96407.0 26 138398.0 27 180389.0 28 180389.0 29 180389.0 30 232294.0 31 284199.0 32 284199.0 33 284199.0 34 318463.0 35 352727.0 36 352727.0 37 352727.0 38 374576.5 39 396426.0 40 396426.0 41 396426.0 42 410737.0 43 425048.0 44 425048.0 45 425048.0 46 431717.5 47 438387.0 48 438387.0 49 438387.0 50 412138.0 51 385889.0 52 385889.0 53 385889.0 54 333517.5 55 281146.0 56 281146.0 57 281146.0 58 235785.5 59 190425.0 60 190425.0 61 190425.0 62 151640.0 63 112855.0 64 112855.0 65 112855.0 66 85448.0 67 58041.0 68 58041.0 69 58041.0 70 41588.0 71 25135.0 72 25135.0 73 25135.0 74 17162.0 75 9189.0 76 9189.0 77 9189.0 78 6178.5 79 3168.0 80 3168.0 81 3168.0 82 2084.0 83 1000.0 84 1000.0 85 1000.0 86 630.5 87 261.0 88 261.0 89 261.0 90 165.0 91 69.0 92 69.0 93 69.0 94 43.5 95 18.0 96 18.0 97 18.0 98 10.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12253108611542665 2 0.004073306236292193 3 0.0013275961066433815 4 0.0035905440156945995 5 0.04022012750353699 6 0.023323449782621224 7 0.017379439941513357 8 0.011888019682215735 9 0.014482866617927798 10 0.020638084930547113 11 0.025586397691672445 12 0.026461404216505578 13 0.024711391166839305 14 0.011586293294342239 15 0.016112189112444674 16 0.014543211895502497 17 0.011556120655554888 18 0.007000052198665103 19 0.0019008762436030233 20 7.241433308963899E-4 21 4.525895818102437E-4 22 0.0028060554072235106 23 0.0020215667987524217 24 0.0014784593005801293 25 1.8103583272409749E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3314261.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.27776116760782 #Duplication Level Percentage of deduplicated Percentage of total 1 80.64466537032024 54.25592536226059 2 12.978441653255892 17.46320995750966 3 3.50740090671532 7.079102415631332 4 1.2407357686640208 3.3389569886514523 5 0.5540117200309154 1.8636334092147766 6 0.27787575335080217 1.121691514092262 7 0.17275971693608158 0.8136020882786461 8 0.11020626872853495 0.5931544821353264 9 0.0812124191400919 0.49174107648756893 >10 0.3863594822302416 4.560013887975773 >50 0.02431037944989394 1.1455227750160062 >100 0.017311457038541672 2.380659979738814 >500 0.002421824993159371 1.1165872684554312 >1k 0.001973338871330484 2.392005941917664 >5k 3.1394027498439514E-4 1.3841928526347549 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7938 0.23951040669398094 No Hit CTGTAGGACGTGGAATATGGCAAGA 7911 0.2386957454467225 No Hit GTCCTACAGTGGACATTTCTAAATT 7308 0.2205016442579507 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6381 0.19253160810207764 No Hit CTTTAGGACGTGAAATATGGCGAGG 6129 0.18492810312766556 No Hit TATCAACGCAGAGTACTTTTTTTTT 5182 0.15635461419604552 No Hit GGTATCAACGCAGAGTACTTTTTTT 5026 0.151647682545219 No Hit GTCCTACAGTGTGCATTTCTCATTT 3881 0.11710001113370372 No Hit GTACATGGGAAGCAGTGGTATCAAC 3673 0.11082410226593499 No Hit CTGAAGGACCTGGAATATGGCGAGA 3486 0.10518181881270063 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.017263878734958E-5 0.0 5 0.0 0.0 0.0 3.017263878734958E-5 0.0 6 0.0 0.0 0.0 3.017263878734958E-5 0.0 7 0.0 0.0 0.0 3.017263878734958E-5 0.0 8 0.0 0.0 0.0 3.017263878734958E-5 0.0 9 0.0 0.0 0.0 3.017263878734958E-5 0.0 10 3.017263878734958E-5 0.0 0.0 3.017263878734958E-5 0.0 11 3.017263878734958E-5 0.0 0.0 3.017263878734958E-5 0.0 12 3.017263878734958E-5 0.0 0.0 6.034527757469916E-5 0.0 13 3.017263878734958E-5 0.0 0.0 6.034527757469916E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGAGG 1525 0.0 12.330519 19 TAGGACC 1175 0.0 12.21109 4 CCAACGA 230 0.0 11.148641 19 GACGTGG 2085 0.0 10.979987 7 AGGACGT 4050 0.0 10.792403 5 GGACGTG 3965 0.0 10.757395 6 AGGACCT 2445 0.0 10.648467 5 GGACCTG 2285 0.0 10.559697 6 TGTAGGA 4065 0.0 10.541725 2 GTAGGAC 3910 0.0 10.449305 3 CTAGTAC 205 3.6379788E-12 10.196815 3 TTAGACG 75 0.0026442844 10.135475 4 TCCAACG 330 0.0 10.0731735 18 CGTGGAA 2250 0.0 10.048131 9 CTGTAGG 4180 0.0 10.034389 1 CCACCTT 1070 0.0 10.031499 13 TAGGACG 4380 0.0 10.022665 4 ACGTGGA 2295 0.0 9.933741 8 TGGCGAG 3805 0.0 9.909415 18 TTAGGAC 2340 0.0 9.907628 3 >>END_MODULE