FastQCFastQC Report
Thu 26 May 2016
SRR1512535_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512535_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847973
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58780.3180782403206107No Hit
GTACATGGGAAGCAGTGGTATCAAC39030.21120438447964338No Hit
GGTATCAACGCAGAGTACTTTTTTT38410.20784935710640795No Hit
TATCAACGCAGAGTACTTTTTTTTT37120.20086873563628904No Hit
GTCCTACAGTGGACATTTCTAAATT32790.17743765736837064No Hit
GTCCTAAAGTGTGTATTTCTCATTT31250.16910420228001166No Hit
CTGTAGGACGTGGAATATGGCAAGA30620.16569506156204664No Hit
CTTTAGGACGTGAAATATGGCGAGG27990.15146325189816084No Hit
CCCATGTACTCTGCGTTGATACCAC27210.14724241100925176No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18640.10086727457598137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG500.001527966813.2671157
TAGGACC5150.012.8806944
TATGTCG905.496677E-712.649446516
GTGCGCC701.11435096E-412.18673511
GACGTGA9750.011.4690077
AGTGCGT752.167337E-411.3462098
TTAGGAC11950.010.9435843
GGACGTG18850.010.8590266
AGGACGT19400.010.84425455
GTGCGTA700.001524476310.8288269
TGTCGAG1053.5979228E-610.82352918
GGCGAGG9250.010.6491719
GTCCTAC17150.010.5805521
GTATTAA2450.010.5227361
GTCTAGA2200.010.3663651
GTATAAT2500.010.3122811
ATGTCGA1201.5500736E-610.27795617
TTTAGGA13350.010.2221312
GTCCTAG2153.6379788E-1210.1462521
GCGCCAC750.002685882610.11597713
Produced by FastQC (version 0.11.5)