FastQCFastQC Report
Thu 26 May 2016
SRR1512535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512535_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847973
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73800.39935648410447555No Hit
GGTATCAACGCAGAGTACTTTTTTT52210.28252577283326114No Hit
TATCAACGCAGAGTACTTTTTTTTT51220.2771685517050303No Hit
GTACATGGGAAGCAGTGGTATCAAC43510.23544716291850587No Hit
GTCCTAAAGTGTGTATTTCTCATTT35630.1928058472715781No Hit
CTGTAGGACGTGGAATATGGCAAGA35260.19080365351658277No Hit
GTCCTACAGTGGACATTTCTAAATT35100.1899378400009091No Hit
CTTTAGGACGTGAAATATGGCGAGG33740.182578425117683No Hit
CCCATGTACTCTGCGTTGATACCAC31600.1709981693455478No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23790.12873564711172727No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20840.11277221041649417No Hit
GAGTACATGGGAAGCAGTGGTATCA19400.10497988877543124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC500.001500732513.2988268
TAGGACC7150.012.0940974
ATAGGAC2100.011.3121993
GGCGAGG8500.011.28418419
CTACCGT855.3096137E-511.1794174
GGACCGG600.005879049711.0826556
AACCGTC700.001492732710.8561847
GCCGGTT700.001492732710.85618411
ATAGCGA803.7820393E-410.68424317
AGGACGT20300.010.53261855
TCGAACT1451.4515535E-810.47899719
GGACGTG20000.010.4018626
TGTAGGA20150.010.3746612
CGGTTTC1106.056336E-610.3621613
GTCCTAA10750.010.3531931
AGGACCT14200.010.3058135
GTAAGAC1951.4551915E-1110.2331893
ACCGGGA750.00264991710.1324388
ACAGCGC750.00264991710.1324388
TGGCGAG20950.010.06348618
Produced by FastQC (version 0.11.5)