FastQCFastQC Report
Thu 26 May 2016
SRR1512534_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512534_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3117271
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT81360.2609975199461324No Hit
GTCCTAAAGTGTGTATTTCTCATTT73010.23421127004998923No Hit
CTGTAGGACGTGGAATATGGCAAGA71780.230265511083252No Hit
GTATCAACGCAGAGTACTTTTTTTT64610.20726462344788119No Hit
CTTTAGGACGTGAAATATGGCGAGG62980.2020356908334245No Hit
GTACATGGGAAGCAGTGGTATCAAC55910.17935559660998354No Hit
GTCCTACAGTGTGCATTTCTCATTT45440.1457685263809274No Hit
GGTATCAACGCAGAGTACTTTTTTT38990.1250773513114516No Hit
CCCATGTACTCTGCGTTGATACCAC38900.12478863724071472No Hit
TATCAACGCAGAGTACTTTTTTTTT37940.12170902048618808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA500.001527572613.26804356
TAGGACC13150.013.1887674
CCAACGA1207.767085E-1012.62931419
ACCGACC604.2540996E-412.6092118
GGATTCG550.00310174412.07411714
ACCGTGC951.0996082E-611.9455678
GGCGAGG21450.011.481194519
GCGTGCG752.1238654E-411.37151059
TCCAACG2450.011.21101618
AGGACCT26550.011.1013245
TGTCGAG1553.3105607E-1010.99901718
GGACCTG25800.010.8730376
GTGTAGG5850.010.8461931
TGTAGGA46050.010.8459322
TGTCCAC12100.010.73139210
AGGACGT46850.010.7212445
GGACGTG46050.010.6605366
AATGTCC11650.010.6339478
GACGTGG22800.010.3916397
ACCCGTG1106.2264808E-610.3387377
Produced by FastQC (version 0.11.5)