FastQCFastQC Report
Thu 26 May 2016
SRR1512534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512534_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3117271
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT88200.28293978932213465No Hit
GTATCAACGCAGAGTACTTTTTTTT84230.2702042908685193No Hit
CTGTAGGACGTGGAATATGGCAAGA82950.2660981351958171No Hit
GTCCTAAAGTGTGTATTTCTCATTT78010.2502509406464821No Hit
CTTTAGGACGTGAAATATGGCGAGG74730.23972891673518282No Hit
GTACATGGGAAGCAGTGGTATCAAC63380.20331886448114392No Hit
TATCAACGCAGAGTACTTTTTTTTT54770.17569855171398316No Hit
GGTATCAACGCAGAGTACTTTTTTT52470.16832030323959643No Hit
GTCCTACAGTGTGCATTTCTCATTT48840.1566755023865426No Hit
CCCATGTACTCTGCGTTGATACCAC45960.1474366521229627No Hit
CTGAAGGACCTGGAATATGGCGAGA38130.1223185279688548No Hit
CTGTAGGACCTGGAATATGGCGAGA36230.11622345314218752No Hit
ATTTAGAAATGTCCACTGTAGGACG31240.10021586188688761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC500.001496989313.3037093
GGACGTA500.001501340313.2985856
TCCAACG2900.013.09974118
TACGACA604.083357E-412.6706054
TGTCGAG1650.012.66308418
CCAACGT1353.7471182E-1011.95938819
GCGTGCG802.8721239E-511.8735469
TAGGACC15250.011.52817254
CCAACGA1900.011.49656319
CGTGCGC855.3332595E-511.17510110
GGCGAGG19550.011.02739319
CCACCTT9650.010.92559813
TGTAGGA50350.010.7954622
CCGTTCA803.777238E-410.6861929
GTGTAGG5200.010.6106891
CTGTAGG49400.010.3988611
AGGACCT28450.010.321315
AATGTCC11200.010.2621368
GTAGGAC51150.010.2550573
GATATAC8300.010.200721
Produced by FastQC (version 0.11.5)