FastQCFastQC Report
Thu 26 May 2016
SRR1512533_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512533_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2695454
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62430.2316121885218594No Hit
GTCCTACAGTGGACATTTCTAAATT58410.21669818887653064No Hit
GTCCTAAAGTGTGTATTTCTCATTT57160.2120607511758687No Hit
CTGTAGGACGTGGAATATGGCAAGA54420.20189548773601773No Hit
CTTTAGGACGTGAAATATGGCGAGG48530.18004388129049873No Hit
GTACATGGGAAGCAGTGGTATCAAC43400.16101183696698218No Hit
GGTATCAACGCAGAGTACTTTTTTT38920.14439126024780982No Hit
TATCAACGCAGAGTACTTTTTTTTT36440.13519058384969657No Hit
GTCCTACAGTGTGCATTTCTCATTT34020.12621250446121507No Hit
CCCATGTACTCTGCGTTGATACCAC32320.11990558918831484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11750.012.8231834
CGTGCGC905.5677265E-712.63665110
GTATTAG2650.011.9726321
GCGCCAC1552.5465852E-1111.62580213
AAGGCGT1800.011.5820866
ATTAGAC1701.2732926E-1111.148533
TAGGACT3750.011.1188014
GATTCGT600.00592685111.07089815
TTCGCAT600.005930145711.07007217
AGGACGT33550.010.9873675
GGACGTG33000.010.883336
TGTAGGA37050.010.8446172
AGGACCT22550.010.8420095
TTAGGAC20100.010.7020343
GTAGGAC36700.010.6898323
GCCTACG650.00775518710.6775171
GGCGAGG15350.010.67509919
GACGTGA17250.010.6024147
TCCAACG1803.8198777E-1110.52367618
GTATAAG3300.010.5157351
Produced by FastQC (version 0.11.5)