FastQCFastQC Report
Thu 26 May 2016
SRR1512533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512533_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2695454
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87940.326253017116968No Hit
CTGTAGGACGTGGAATATGGCAAGA66080.24515350660779223No Hit
GTCCTAAAGTGTGTATTTCTCATTT64140.2379562032963649No Hit
GTCCTACAGTGGACATTTCTAAATT62820.23305906908446591No Hit
CTTTAGGACGTGAAATATGGCGAGG59460.22059363654508665No Hit
TATCAACGCAGAGTACTTTTTTTTT58400.21666108937492531No Hit
GGTATCAACGCAGAGTACTTTTTTT58190.21588199984121414No Hit
GTACATGGGAAGCAGTGGTATCAAC53500.19848233358833056No Hit
CCCATGTACTCTGCGTTGATACCAC36700.13615517089143425No Hit
GTCCTACAGTGTGCATTTCTCATTT35870.1330759122581947No Hit
CTGAAGGACCTGGAATATGGCGAGA30040.11144690282230749No Hit
CTGTAGGACCTGGAATATGGCGAGA28090.10421250000927489No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGT400.005280808514.24984711
TAGGACC11250.012.2456774
GGCGAGG15550.011.237719
TGTAGGA35950.011.0990412
GGACCTG22250.010.7592366
AGGACGT38750.010.6165525
GGACGTG38150.010.6078156
AGGACCT23200.010.5657185
GACGTGG18300.010.5379437
CTGTAGG36750.010.4825031
GTAGGAC37050.010.3337973
TTAGGAC23050.010.2217183
GACGTGA21050.010.1992257
TGGCGAG35500.010.1125718
ACTGCGG856.5980654E-410.0585298
CGTGGAA19600.09.9845869
ACGTGGA19800.09.8835498
TAGGACG41900.09.7957344
GTCCTAC34700.09.7860181
TGGCAAG30450.09.63760918
Produced by FastQC (version 0.11.5)