FastQCFastQC Report
Thu 26 May 2016
SRR1512532_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512532_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367538
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT69110.2919066135369316No Hit
GTCCTAAAGTGTGTATTTCTCATTT67160.2836702093060386No Hit
CTGTAGGACGTGGAATATGGCAAGA64060.27057643847743945No Hit
CTTTAGGACGTGAAATATGGCGAGG58720.248021362275917No Hit
GTATCAACGCAGAGTACTTTTTTTT57050.24096762121663937No Hit
GTCCTACAGTGTGCATTTCTCATTT36560.15442201983664042No Hit
GTACATGGGAAGCAGTGGTATCAAC35080.1481708002152447No Hit
GGTATCAACGCAGAGTACTTTTTTT34170.14432714490749463No Hit
TATCAACGCAGAGTACTTTTTTTTT33880.14310224376546438No Hit
CTGAAGGACCTGGAATATGGCGAGA31010.13097994625640644No Hit
GTCCTTCAGTGTGCATTTCTCATTT26870.11349342650466435No Hit
ATTTAGAAATGTCCACTGTAGGACG25630.10825591817322468No Hit
CTGTAGGACCTGGAATATGGCGAGA24990.10555268806667517No Hit
CCCATGTACTCTGCGTTGATACCAC24720.10441226286547461No Hit
TTTCTAAATTTTCCACCTTTTTCAG24100.10179350869975476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGG307.8511296E-418.9540586
TCCAACG1950.014.0820918
GTATACG450.00866440413.2227621
CCAACGT802.0724801E-613.02151919
TAGGACC10800.012.8992894
CCAACGA1403.6379788E-1212.85240919
GGCGAGG18650.012.0852919
TGGCGGG752.1386334E-411.3627918
GACGTGA23350.011.1613847
GTGTAGG4650.011.0900591
ACGCCCT600.00598403511.0565345
AGGACCG600.00598403511.0565345
GGACGTG41000.011.0257146
AATGTCC10950.010.9671878
AGGACGT41050.010.9661115
GGACCTG23950.010.8817666
GTACCGT700.00152232910.830896
GTCTTAG2850.010.7869911
GTCCTAA21150.010.7845231
TGTCCAC11850.010.71868710
Produced by FastQC (version 0.11.5)