FastQCFastQC Report
Thu 26 May 2016
SRR1512532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512532_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367538
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA76520.3232049496143251No Hit
GTCCTACAGTGGACATTTCTAAATT75880.32050171950777556No Hit
GTCCTAAAGTGTGTATTTCTCATTT73520.3105335584898743No Hit
GTATCAACGCAGAGTACTTTTTTTT71160.300565397471973No Hit
CTTTAGGACGTGAAATATGGCGAGG68700.2901748567499233No Hit
TATCAACGCAGAGTACTTTTTTTTT50250.2122458013345509No Hit
GGTATCAACGCAGAGTACTTTTTTT47350.1999967899142485No Hit
GTACATGGGAAGCAGTGGTATCAAC40900.17275329899667924No Hit
GTCCTACAGTGTGCATTTCTCATTT39770.1679804083398028No Hit
CTGAAGGACCTGGAATATGGCGAGA37870.15995519396098395No Hit
ATTTAGAAATGTCCACTGTAGGACG31070.13123337407889546No Hit
CTGTAGGACCTGGAATATGGCGAGA29980.12662943530367834No Hit
TTTCTAAATTTTCCACCTTTTTCAG29350.12396844316754367No Hit
CCCATGTACTCTGCGTTGATACCAC29230.12346158752256564No Hit
GTCCTTCAGTGTGCATTTCTCATTT28400.11995583597813424No Hit
GAATATGGCAAGAAAACTGAAAATC27720.1170836539899254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13050.013.4711334
CCAACGA1251.03682396E-1012.91372419
CGGTAGG751.4608215E-512.6822091
TCCAACG1950.012.17485718
CGAAATC2850.011.66518813
GGCGAGG18350.011.64284319
AATGTCC10150.011.4182378
AGGACCT26300.011.2012695
TATCCGT600.005877197711.0833336
ACGAAAT3000.011.08263112
GGACCTG25200.010.9702386
AAATGTC10700.010.9203317
AGTCGTC3050.010.9011788
TGTAGGA44000.010.8843482
CCACCTT9700.010.86975613
CACCTTT10000.010.82748414
ACTGTTC8150.010.7234738
TAGAAAT11900.010.7004184
GGCGAAA1252.2565655E-710.63483119
AGGACGT44350.010.6279465
Produced by FastQC (version 0.11.5)