FastQCFastQC Report
Thu 26 May 2016
SRR1512531_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512531_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502918
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43440.28903772527842503No Hit
GTCCTACAGTGGACATTTCTAAATT28940.19255874239313123No Hit
CTGTAGGACGTGGAATATGGCAAGA27950.18597155666510082No Hit
GTCCTAAAGTGTGTATTTCTCATTT27020.1797835943145268No Hit
GGTATCAACGCAGAGTACTTTTTTT26130.17386178088225707No Hit
TATCAACGCAGAGTACTTTTTTTTT25440.16927071204150856No Hit
CTTTAGGACGTGAAATATGGCGAGG22140.14731342628140726No Hit
GTCCTACAGTGTGCATTTCTCATTT18490.12302733748614363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTCA400.00535317714.22004812
GGTATCA7150.012.7673141
TAGGACC6100.012.2741354
GTCCTAC15500.012.162971
GTATAAC1705.456968E-1211.6734381
TTAGAAC1850.011.2705353
GTCCTAA9450.010.9199151
GGCGAGG8500.010.91677619
GTACTAG1001.4824151E-510.9146651
TGTCCAC5300.010.90988510
AAGGCGT1053.565181E-610.8314256
AATGTCC4900.010.805778
GTTTAGG2400.010.7492911
AAATGTC4850.010.7476767
CCTACAG18750.010.6653423
TCCTACA18250.010.6455982
GTCTTAG2150.010.6146841
TGGCGAG16250.010.60380418
AGGACCT10950.010.472855
AGGACGT17800.010.4358955
Produced by FastQC (version 0.11.5)