FastQCFastQC Report
Thu 26 May 2016
SRR1512531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512531_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1502918
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57320.3813913999300029No Hit
TATCAACGCAGAGTACTTTTTTTTT37790.2514441905679485No Hit
GGTATCAACGCAGAGTACTTTTTTT36920.24565545159483085No Hit
CTGTAGGACGTGGAATATGGCAAGA33330.22176858617702364No Hit
GTCCTACAGTGGACATTTCTAAATT31180.20746308181816975No Hit
GTCCTAAAGTGTGTATTTCTCATTT28960.19269181685228337No Hit
CTTTAGGACGTGAAATATGGCGAGG25960.17273064797946394No Hit
GTCCTACAGTGTGCATTTCTCATTT19600.1304129699690868No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17750.11810358249751483No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15200.10113658895561835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGCG250.00603522118.9982387
GGTATCA9400.012.7498341
CCTATAC1657.2759576E-1211.5179163
CTATACT1900.011.5035274
TCTATAC2100.010.8597483
CCTCGAA1053.4747536E-610.8546917
TAGGACC5950.010.700764
GTCCTAC17800.010.6339571
AGGCGTG909.522634E-510.5545757
AGGACGT16750.010.3826325
TGTAGGA18400.010.3802092
AATGTCC4950.010.3626758
TCCTACA19300.010.339272
GGACGTG16950.010.1432636
GTCCTAA8100.010.09892751
GTAGGAC17700.010.0928183
CCTACAG19250.010.0699493
TATACTG2850.010.00345
TAAAGGT2201.8189894E-129.9348644
CTGTAGG19150.09.9339741
Produced by FastQC (version 0.11.5)