FastQCFastQC Report
Thu 26 May 2016
SRR1512530_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512530_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141995
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57860.2701220124229982No Hit
TATCAACGCAGAGTACTTTTTTTTT34960.1632123324284137No Hit
GGTATCAACGCAGAGTACTTTTTTT34790.1624186797821657No Hit
GTCCTACAGTGGACATTTCTAAATT29420.13734859325068452No Hit
GTCCTAAAGTGTGTATTTCTCATTT28540.1332402736701066No Hit
CTGTAGGACGTGGAATATGGCAAGA26790.12507031995873005No Hit
CTTTAGGACGTGAAATATGGCGAGG24860.11606002815132622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG500.001526630613.2688145
CCAACGA1001.0522854E-813.25917219
TATACCG550.003123517612.0625585
ACCTCGC550.003181591212.0329188
TCCAACG1701.8189894E-1211.69789818
GCGCCAC1201.3069621E-711.06409413
TAGGACC7050.010.88934
CTACGGC700.001521293810.8316853
AATGTCC6050.010.7827458
CGTGCGC1106.2087875E-610.34079210
GGCGAGG8000.010.29953619
GTATTAA2750.010.0888581
GGTATCA14500.010.0453711
GGACCTG12550.010.0441226
AAATGTC5800.09.9679527
GTCCTAC18550.09.9354081
TGGCAAG15350.09.93241818
GATATAC5800.09.9086991
CTAATAC2500.09.8568343
GTCCTAA9050.09.8539561
Produced by FastQC (version 0.11.5)