FastQCFastQC Report
Thu 26 May 2016
SRR1512530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512530_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141995
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72650.3391697926465748No Hit
TATCAACGCAGAGTACTTTTTTTTT51220.2391228737695466No Hit
GGTATCAACGCAGAGTACTTTTTTT49210.2297390983639084No Hit
GTCCTACAGTGGACATTTCTAAATT31960.14920669749462534No Hit
CTGTAGGACGTGGAATATGGCAAGA31150.14542517606250246No Hit
GTCCTAAAGTGTGTATTTCTCATTT30520.1424839927264069No Hit
CTTTAGGACGTGAAATATGGCGAGG28040.13090600118114187No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22540.10522900380252988No Hit
GTACATGGGAAGCAGTGGTATCAAC21570.1007005151739383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG2750.013.46693319
TAGGACC7200.012.4055534
ACTGTTC6100.012.1474228
GTGCCGC550.003069322812.0907911
GGTATCA18150.011.2143491
GATATAC7350.011.1287921
TTAGGAC12050.011.0382673
GGCGAGG9050.011.01735919
TGTCGAG1158.8615343E-710.73526418
ACACCGT803.773084E-410.6871466
GACGTGA9950.010.5978877
AATGTCC5750.010.573818
GGACGAG909.521212E-510.5552056
CACTGTT7100.010.4365177
AGGACCT14350.010.3965395
TGGCGAG20700.010.23061518
GTCCTAC18600.010.1761
AAGAGCG856.5787317E-410.0615445
CTGAGCG856.595706E-410.0587249
GGACGTG17450.010.016866
Produced by FastQC (version 0.11.5)