FastQCFastQC Report
Thu 26 May 2016
SRR1512529_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512529_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464098
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53750.21813255803949358No Hit
GTACATGGGAAGCAGTGGTATCAAC34410.1396454199467716No Hit
TATCAACGCAGAGTACTTTTTTTTT33210.13477548376728524No Hit
GGTATCAACGCAGAGTACTTTTTTT32680.1326245952880121No Hit
GTCCTACAGTGGACATTTCTAAATT29830.12105849686173197No Hit
GTCCTAAAGTGTGTATTTCTCATTT27200.11038522006835767No Hit
CTGTAGGACGTGGAATATGGCAAGA26670.10823433158908453No Hit
CCCATGTACTCTGCGTTGATACCAC25080.10178166615126508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6350.012.5379834
GTCGAGG1103.9948645E-812.05150819
GACCGCT907.65945E-611.5843667
TGTCGAG1157.414019E-811.52682518
GTCCTAT2750.011.17246151
ACCGCTA600.006087854511.0310348
TTAGGAC12650.011.0140983
CGTGCGC1053.5610592E-610.8332410
TATGTCG1158.983934E-710.72401716
GGACCGC1159.0883805E-710.7143956
GCGTGCG1159.114665E-710.7119919
GTCCTAC15700.010.5423321
CTAGGAC3250.010.4988383
GACGTGA9500.010.4758157
GGCGTGC1451.5548721E-810.4332938
ATAAGAC2001.8189894E-1210.4259293
TAGGACA6900.010.1649384
GGACGTG16800.010.0987096
GGCGAGG10000.010.03718519
TGGCGAG19100.09.96419418
Produced by FastQC (version 0.11.5)