FastQCFastQC Report
Thu 26 May 2016
SRR1512529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512529_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464098
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72130.2927237471886264No Hit
TATCAACGCAGAGTACTTTTTTTTT49470.20076311899932553No Hit
GGTATCAACGCAGAGTACTTTTTTT45790.18582864804890065No Hit
GTACATGGGAAGCAGTGGTATCAAC38550.15644669976599956No Hit
CTGTAGGACGTGGAATATGGCAAGA31600.12824165272647436No Hit
GTCCTACAGTGGACATTTCTAAATT31040.12596901584271405No Hit
CCCATGTACTCTGCGTTGATACCAC28640.1162291434837413No Hit
CTTTAGGACGTGAAATATGGCGAGG28380.11517399064485259No Hit
GTCCTAAAGTGTGTATTTCTCATTT27900.11322601617305805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG400.005239745614.2665551
TAGGACC8250.011.4025834
GGCGAGG8100.010.78577919
CGTGCGC909.522954E-510.555160510
TGGCGAG17050.010.52697918
GTAGGAC19350.010.5084143
TGTAGGA19950.010.4781392
AGGACGT18500.010.324175
GGACGTG18500.010.1160446
TAGGACG19600.010.0354374
CTGTAGG20150.09.9122461
TATGGCG19200.09.7945216
GTCCTAC17550.09.6465221
GACGTGA10250.09.6386647
TTAGGAC12100.09.5017663
GGACCGC1002.7567803E-49.4998376
GACGTGG9600.09.4996447
TCCTACA19100.09.452022
GGCGAGA10350.09.45029919
ATGGCGA19800.09.44955617
Produced by FastQC (version 0.11.5)