FastQCFastQC Report
Thu 26 May 2016
SRR1512528_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512528_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751444
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43060.24585427795578962No Hit
GTCCTACAGTGGACATTTCTAAATT36720.2096555756278819No Hit
GTCCTAAAGTGTGTATTTCTCATTT35440.20234732026830432No Hit
CTGTAGGACGTGGAATATGGCAAGA33760.19275523510885872No Hit
CTTTAGGACGTGAAATATGGCGAGG28710.1639218838855253No Hit
GGTATCAACGCAGAGTACTTTTTTT27180.15518623490103023No Hit
TATCAACGCAGAGTACTTTTTTTTT25470.14542286250659456No Hit
GTCCTACAGTGTGCATTTCTCATTT22820.1302924900824691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7200.012.1086894
GTTCTAG1705.456968E-1211.6600441
CCGGTTT855.437975E-511.15314312
AAGGCCG600.00598609811.0557585
GGCGAGG8450.010.98655919
TGTAGGA23700.010.8361542
GACGTGG10650.010.6775937
TATGTCG1605.5661076E-1010.6737716
GTCCTAC19900.010.6580991
AGGACGT22050.010.6152475
GGACGTG21600.010.5292946
TGTCGAG1355.948459E-810.52325818
CTAGGAC1909.094947E-1210.4738763
CTGTAGG21800.010.4565981
GTAGGAC23550.010.42198853
GTGTAGG3700.010.1789031
GTCCAGG2750.010.0912381
GTCCTAA12300.010.0721921
TCCTACA23650.010.0576562
ATGTCGA1701.5934347E-910.045917
Produced by FastQC (version 0.11.5)