FastQCFastQC Report
Thu 26 May 2016
SRR1512528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512528_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751444
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54140.30911636341213306No Hit
CTGTAGGACGTGGAATATGGCAAGA39620.2262133416769249No Hit
TATCAACGCAGAGTACTTTTTTTTT38460.21959023525730767No Hit
GGTATCAACGCAGAGTACTTTTTTT38130.21770607567241657No Hit
GTCCTACAGTGGACATTTCTAAATT38080.2174205969474331No Hit
GTCCTAAAGTGTGTATTTCTCATTT35770.2042314798531954No Hit
CTTTAGGACGTGAAATATGGCGAGG33520.19138493722893796No Hit
GTCCTACAGTGTGCATTTCTCATTT22980.1312060220024163No Hit
CTGAAGGACCTGGAATATGGCGAGA19080.10893868145370335No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18770.10716871335880564No Hit
CTGTAGGACCTGGAATATGGCGAGA18250.10419973461897726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6650.014.1457914
AATCCCG1650.012.08651219
TAGGACT3050.011.5269824
AGGACGG752.069559E-411.4026915
ATGTCGA752.0794646E-411.39683117
TTAGGAC13400.011.3456373
AATGTCC5300.011.1123388
AGGACCT12900.011.0491195
AGGACGA700.001488631510.8597065
CGGTTTC803.7759062E-410.68605313
TATGTCG803.7778995E-410.68544416
TGTCGAG803.7818888E-410.68422318
GGACCTG13000.010.5222556
GTAGGAC22950.010.5163433
TGTAGGA23450.010.4947142
GGTATCA15300.010.4451741
GATATAC4200.010.4185281
GGCGAGG10850.010.3280419
AAATGTC5250.010.3134797
GGACGTG22600.010.2978686
Produced by FastQC (version 0.11.5)