FastQCFastQC Report
Thu 26 May 2016
SRR1512527_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512527_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951994
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56810.2910357306426147No Hit
GGTATCAACGCAGAGTACTTTTTTT35920.18401695906852172No Hit
GTACATGGGAAGCAGTGGTATCAAC35210.18037965280630985No Hit
TATCAACGCAGAGTACTTTTTTTTT34170.17505176757715443No Hit
GTCCTACAGTGGACATTTCTAAATT32660.16731608806174608No Hit
GTCCTAAAGTGTGTATTTCTCATTT30190.15466236064250197No Hit
CTGTAGGACGTGGAATATGGCAAGA29700.15215210702491913No Hit
CTTTAGGACGTGAAATATGGCGAGG26220.13432418337351448No Hit
CCCATGTACTCTGCGTTGATACCAC25520.13073810677696757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6100.011.8086394
TAGAACG658.182691E-411.6652144
CCAACGA658.228749E-411.6574119
GCCAGTA1201.3172757E-711.057652
GGCGAGG8650.010.9498819
AGGACCT12600.010.9072075
AGGACGT18000.010.8996855
TGTAGGA17950.010.7716382
GTATAGG2300.010.7679321
GGACGTG18250.010.7503736
TGTCGAG1159.183186E-710.70542518
GTTTAGG1957.2759576E-1210.6685351
GTCCTAC17100.010.6596221
TTAGGAC12450.010.6579763
GTTCTAG2051.8189894E-1210.6313611
GGACCTG11250.010.53109556
GTAGGAC17650.010.2029313
TCCTACA19400.010.1131092
GCCGAAA750.002695495510.11167412
GCACCGT750.002699528110.1098526
Produced by FastQC (version 0.11.5)