FastQCFastQC Report
Thu 26 May 2016
SRR1512527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512527_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951994
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69130.35415067874184036No Hit
TATCAACGCAGAGTACTTTTTTTTT48990.2509741320926191No Hit
GGTATCAACGCAGAGTACTTTTTTT47060.24108680661928264No Hit
GTACATGGGAAGCAGTGGTATCAAC41660.21342278716020643No Hit
CTGTAGGACGTGGAATATGGCAAGA34890.17874030350503126No Hit
GTCCTACAGTGGACATTTCTAAATT34420.17633250921877833No Hit
GTCCTAAAGTGTGTATTTCTCATTT33610.1721829062999169No Hit
CTTTAGGACGTGAAATATGGCGAGG31390.16080992052229667No Hit
CCCATGTACTCTGCGTTGATACCAC29720.15225456635624904No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21830.11183436014659881No Hit
GTCCTACAGTGTGCATTTCTCATTT20290.10394499163419559No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20180.10338146531188108No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGG500.001495371113.3052055
GCGTGCG550.003070541212.0900619
GTCTAAG1406.6211214E-1011.5553381
TAGGACC5550.011.4726624
TGGATCG752.0668078E-411.4044625
TCTATAC2350.011.3218373
GTATAGA1900.011.0187131
AAGTCGT2150.010.6039087
CGTGCGC909.52378E-510.55481510
GGCGAGG10100.010.529057519
TGTAGGA17450.010.1829342
GGACGTG19500.010.1323626
GCGCCAC750.002651884710.13158413
AGGACGT19800.010.07975
AATGTCC5950.010.0581188
GACGTGA11550.010.0339287
AGTCGTC2300.09.9123488
GTCCTAC18050.09.8588481
GACAGCG1356.449609E-79.8511617
ACTGGTC1856.493792E-109.7560738
Produced by FastQC (version 0.11.5)