FastQCFastQC Report
Thu 26 May 2016
SRR1512526_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512526_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3374358
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79240.23482985504205542No Hit
GTATCAACGCAGAGTACTTTTTTTT70280.20827665588535657No Hit
CTGTAGGACGTGGAATATGGCAAGA70120.20780249161470124No Hit
GTCCTAAAGTGTGTATTTCTCATTT66310.19651145491972102No Hit
GTACATGGGAAGCAGTGGTATCAAC60670.1797971643791204No Hit
CTTTAGGACGTGAAATATGGCGAGG55220.16364594390992301No Hit
GTCCTACAGTGTGCATTTCTCATTT43880.13003955122722605No Hit
CCCATGTACTCTGCGTTGATACCAC42690.12651295446422697No Hit
TATCAACGCAGAGTACTTTTTTTTT42330.12544608485525247No Hit
GGTATCAACGCAGAGTACTTTTTTT40630.1204080894795395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC15200.013.7810824
TCTACGC655.5739692E-513.1239273
TACGGCG550.003101681612.07419716
TGTAGGA48200.011.3268752
GTCCTAC40700.011.2863581
GGCGAGG19250.011.26703719
CACCTTT10800.011.24436714
GTAGGAC46450.011.0598253
TCCTACA46050.011.0118122
GGACCTG27550.011.0093856
AGGACCT29350.010.9477855
CCACCTT11150.010.88702213
GTGTAGG6650.010.8675441
CGTGCGC1053.5597404E-610.833899510
AATCCCG5250.010.82422719
GGACGTG42150.010.6146
AGGACGT43600.010.5436245
CTGTAGG43850.010.5433231
CCTACAG45800.010.5338463
TGGCGAG44700.010.48621218
Produced by FastQC (version 0.11.5)