FastQCFastQC Report
Thu 26 May 2016
SRR1512526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512526_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3374358
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT91840.27217029135616316No Hit
CTGTAGGACGTGGAATATGGCAAGA85350.25293700312770606No Hit
GTCCTACAGTGGACATTTCTAAATT84380.25006238223685806No Hit
GTACATGGGAAGCAGTGGTATCAAC72760.21562620208051428No Hit
GTCCTAAAGTGTGTATTTCTCATTT71890.21304793385882587No Hit
CTTTAGGACGTGAAATATGGCGAGG68680.20353501317880318No Hit
TATCAACGCAGAGTACTTTTTTTTT61710.1828792321383801No Hit
GGTATCAACGCAGAGTACTTTTTTT58940.1746702632026596No Hit
CCCATGTACTCTGCGTTGATACCAC50240.14888758098577565No Hit
GTCCTACAGTGTGCATTTCTCATTT49110.14553879582427234No Hit
CTGAAGGACCTGGAATATGGCGAGA39640.11747419805485963No Hit
CTGTAGGACCTGGAATATGGCGAGA39470.11697039851728831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14600.013.47188954
TCCAACG4000.012.58406418
ACGTTGA855.3304673E-511.1757388
CCAACGA2550.011.17292319
AGGACCT27900.011.136655
TGTAGAA17500.011.0218922
AGGACGT43550.010.8437445
GATATAC13350.010.7594451
TGTAGGA47850.010.7427012
GGACGTG43000.010.7363476
GGCGAGG17900.010.66421119
GTAGGAC47050.010.501273
TTAGGAC25250.010.4612023
AGGCCGT1002.4027891E-510.449166
GTCCTAC41800.010.4455341
GGACCTG28100.010.4119756
TAGAACG1106.0363236E-610.3657234
GAACAGT9300.010.3163766
GACGTGG23350.010.2926877
CTGTAGG47950.010.1770751
Produced by FastQC (version 0.11.5)