FastQCFastQC Report
Thu 26 May 2016
SRR1512525_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512525_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008995
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58380.2905930577228913No Hit
GGTATCAACGCAGAGTACTTTTTTT37240.18536631499829515No Hit
GTACATGGGAAGCAGTGGTATCAAC36290.18063758247282846No Hit
TATCAACGCAGAGTACTTTTTTTTT35990.17914429851741792No Hit
GTCCTACAGTGGACATTTCTAAATT32330.1609262342614093No Hit
CTGTAGGACGTGGAATATGGCAAGA30470.15166787373786395No Hit
GTCCTAAAGTGTGTATTTCTCATTT28240.14056779633597893No Hit
CCCATGTACTCTGCGTTGATACCAC24690.12289726953028753No Hit
CTTTAGGACGTGAAATATGGCGAGG24480.12185197076150014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC2550.012.4306961
TAGGACC5900.012.0472654
CTAGGAC2650.011.8017733
GTATTAG2400.011.55666351
TGCGTGA600.005971650611.05947910
GGCGAGG7800.010.92892519
GTCCTAA8750.010.867981
CTACACT2600.010.5707034
GTCCTAC15150.010.5268611
GTGTTAG2200.010.355971
TTAGGAC11550.010.1746373
TATGTCG750.00267637710.12038216
TCCTAAA10450.010.066672
GGACGTG18700.09.9839826
TCCTACA17950.09.978762
ACTGTTC3800.09.9515938
AGGACGT19250.09.9448865
CTAAAGT11000.09.9079634
CCTACAG17850.09.8753823
TTTAGGA12750.09.811672
Produced by FastQC (version 0.11.5)