FastQCFastQC Report
Thu 26 May 2016
SRR1512525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512525_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008995
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75070.3736694217755644No Hit
GGTATCAACGCAGAGTACTTTTTTT53060.2641121555802777No Hit
TATCAACGCAGAGTACTTTTTTTTT51770.25769103457201237No Hit
GTACATGGGAAGCAGTGGTATCAAC41110.20462967802309115No Hit
CTGTAGGACGTGGAATATGGCAAGA35990.17914429851741792No Hit
GTCCTACAGTGGACATTTCTAAATT33570.16709847461043953No Hit
GTCCTAAAGTGTGTATTTCTCATTT30470.15166787373786395No Hit
CCCATGTACTCTGCGTTGATACCAC29450.1465907082894681No Hit
CTTTAGGACGTGAAATATGGCGAGG29370.14619249923469196No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24270.12080667199271278No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21590.10746666865771193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTC653.3779488E-614.61344913
TAGCACC1251.4042598E-912.1635394
TAGTACG550.003062300812.0944284
GGCGAGG8550.010.99498619
TCGAACT1053.4862478E-610.85219319
TAGGACC5650.010.5960034
TGTAGGA18400.010.2255472
TGGCGAG17300.010.21023318
GTATAAA2900.010.1713021
GGACGTG19100.010.1455086
AGGACGT19350.010.0680315
TAATACT1909.822543E-1110.0029114
GACGTGA10100.09.9692257
CTATTAG1054.0348496E-59.9681881
GTAATAC1054.093846E-59.95502953
GGACTTA1151.0279295E-59.9119796
GTAGGAC18450.09.8888093
ACGTGAA11350.09.79198
TAGGACG20800.09.7768824
CTGTAGG18400.09.7736511
Produced by FastQC (version 0.11.5)