FastQCFastQC Report
Thu 26 May 2016
SRR1512524_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512524_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470355
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT60000.2428800718925013No Hit
GTCCTACAGTGGACATTTCTAAATT59770.24194903161691336No Hit
GTATCAACGCAGAGTACTTTTTTTT54250.21960406500280324No Hit
CTGTAGGACGTGGAATATGGCAAGA53290.21571798385252322No Hit
CTTTAGGACGTGAAATATGGCGAGG47350.1916728567351656No Hit
GTCCTACAGTGTGCATTTCTCATTT35360.14313732236864743No Hit
GGTATCAACGCAGAGTACTTTTTTT33980.1375510807151199No Hit
TATCAACGCAGAGTACTTTTTTTTT32230.13046707861825527No Hit
GTACATGGGAAGCAGTGGTATCAAC27780.1124534732862281No Hit
CTGAAGGACCTGGAATATGGCGAGA25110.10164531008701179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG450.00875213813.2026441
TAGGACC12300.012.5607474
GTGTAGG5200.012.3774791
AGGACCT22900.011.7962055
GGCGAGG17350.011.62584419
GGACCTG22700.011.5243436
CCGGTTT1407.021299E-1011.51364712
GTCCTAA18750.011.1958431
GTCTTAG3300.011.1022231
ATACGGC600.005940024711.06753715
GACCGAG600.00597793311.058087
ATAGACG600.00597793311.058083
AATGTCC9500.010.8504428
GGACGTA700.001520594510.8324056
TAGAAAT9500.010.7753934
TGGCGAG36600.010.7631418
GCCGGTT1605.657057E-1010.66488511
TCCAACG2400.010.6531818
ATTTAGA9600.010.3145651
ACGAAAT2500.010.24037412
Produced by FastQC (version 0.11.5)