FastQCFastQC Report
Thu 26 May 2016
SRR1512524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512524_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470355
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71300.28862248543225566No Hit
GTCCTAAAGTGTGTATTTCTCATTT64000.25907207668533466No Hit
CTGTAGGACGTGGAATATGGCAAGA63230.2559551157627143No Hit
GTCCTACAGTGGACATTTCTAAATT63100.25542887560694716No Hit
CTTTAGGACGTGAAATATGGCGAGG56340.22806438750705868No Hit
TATCAACGCAGAGTACTTTTTTTTT48250.1953160578135531No Hit
GGTATCAACGCAGAGTACTTTTTTT47780.1934134972503952No Hit
GTCCTACAGTGTGCATTTCTCATTT38310.15507892590336206No Hit
GTACATGGGAAGCAGTGGTATCAAC32890.13313875940907277No Hit
CTGAAGGACCTGGAATATGGCGAGA31330.12682387753986774No Hit
CTGTAGGACCTGGAATATGGCGAGA29900.1210352358264298No Hit
ATTTAGAAATGTCCACTGTAGGACG27040.1094579523995539No Hit
TTTCTAAATTTTCCACCTTTTTCAG25600.10362883067413386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1350.014.06947118
TAGGACC12150.012.67021754
CTGCGGA550.003071261312.0898919
CCAACGA1052.7359056E-711.75734419
GGCGAGG15850.011.32368319
AAATGTC9000.010.44912057
GGACCTG21500.010.2942736
CACCGTG1201.5262394E-610.2908017
ATTTAGA10400.010.2431811
TGGCGAG36650.010.20948418
AGGACCT22900.010.2081015
TGTAGGA38200.010.1983672
TTAGGAC20600.010.1941923
TTTAGAA11650.010.1136032
AGGACGT34200.010.0861615
GTCCTAA18600.010.0740351
AATGTCC9750.010.0350528
GGACGTG34550.010.0076386
GTACTAG2105.456968E-129.9644551
ACCTTTT9750.09.93682115
Produced by FastQC (version 0.11.5)