FastQCFastQC Report
Thu 26 May 2016
SRR1512523_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512523_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346096
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60570.2581735785747898No Hit
GTCCTACAGTGGACATTTCTAAATT50880.21687092088303295No Hit
GTACATGGGAAGCAGTGGTATCAAC49320.21022157661067575No Hit
GTCCTAAAGTGTGTATTTCTCATTT48770.20787725651465241No Hit
CTGTAGGACGTGGAATATGGCAAGA44760.1907850318145549No Hit
CTTTAGGACGTGAAATATGGCGAGG40890.1742895431388997No Hit
GGTATCAACGCAGAGTACTTTTTTT36130.15400051830786124No Hit
CCCATGTACTCTGCGTTGATACCAC35200.15003648614549447No Hit
TATCAACGCAGAGTACTTTTTTTTT33660.14347238987662908No Hit
GTCCTACAGTGTGCATTTCTCATTT26190.11163226057245738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2000.013.73388618
CCAACGT1051.4351826E-913.53121219
TAGGACC8450.013.45949654
GTGTAGG4450.012.4648731
AATCCCG2050.011.55103519
GGCGAGG13150.011.52468119
AATGTCC8050.011.5136978
GTCCTAA15500.011.311011
AAATGTC8450.011.2162487
CGGTTTC2200.011.20839413
GAAGCGT600.005980713811.0573516
CTGGTCG600.0059921611.0545119
GTCCTAC28550.010.8592061
GGACGTG29850.010.8271556
AGGACGT31000.010.7618095
GACGTGA16450.010.6588457
GCCGGTT2050.010.6335511
TAGAAAT9900.010.6265464
GTCCTAG3100.010.5441641
GTACTAA1603.106834E-910.5241941
Produced by FastQC (version 0.11.5)