FastQCFastQC Report
Thu 26 May 2016
SRR1512523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512523_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346096
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75820.3231751812372554No Hit
GTACATGGGAAGCAGTGGTATCAAC59150.25212097032687497No Hit
GTCCTACAGTGGACATTTCTAAATT55440.2363074656791538No Hit
CTGTAGGACGTGGAATATGGCAAGA54970.2343041375970975No Hit
TATCAACGCAGAGTACTTTTTTTTT52440.22352026515539006No Hit
GTCCTAAAGTGTGTATTTCTCATTT51630.22006772101397384No Hit
GGTATCAACGCAGAGTACTTTTTTT50440.21499546480621426No Hit
CTTTAGGACGTGAAATATGGCGAGG49530.2111166806473392No Hit
CCCATGTACTCTGCGTTGATACCAC40660.1733091910987445No Hit
CTGAAGGACCTGGAATATGGCGAGA27760.11832422884656042No Hit
GTCCTACAGTGTGCATTTCTCATTT27200.11593728474879117No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24790.10566490032803433No Hit
GAGTACATGGGAAGCAGTGGTATCA24630.10498291630010026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC250.006035827618.9984446
AGTACGG400.005269597314.2543035
GCGTGCG957.402923E-812.9986589
CGTGCGC1102.75395E-912.95320810
TAGGACC9650.012.6048414
CTAGGAC2650.012.5506923
GTACGGC550.00307113112.0899186
GCGCCAC1354.7421054E-911.25761713
GACCTCG600.00588262211.0819537
CAGTACG700.001488139710.8604214
ACTTCGT700.001493443710.8557918
GGCGAGG13550.010.372437519
TGTCCAC9300.010.31613910
GGCGTGC1752.3646862E-1010.3130018
GTCCTAC27950.010.2107481
ATTTAGA9000.010.1472141
GTATGAC1502.4356268E-810.1361773
AACCGTG750.0026511710.1320717
AGGACCT20000.010.1205555
TCCAACG1606.206392E-910.09077318
Produced by FastQC (version 0.11.5)