##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512523_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2346096 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.615539176572483 33.0 31.0 34.0 30.0 34.0 2 31.897681936289054 33.0 31.0 34.0 30.0 34.0 3 32.06220589438795 34.0 31.0 34.0 30.0 34.0 4 35.54134187177336 37.0 35.0 37.0 33.0 37.0 5 35.031876359705656 37.0 35.0 37.0 32.0 37.0 6 35.26316612789928 37.0 35.0 37.0 33.0 37.0 7 35.22519581466402 37.0 35.0 37.0 33.0 37.0 8 35.2035377068969 37.0 35.0 37.0 33.0 37.0 9 36.80619974630194 39.0 37.0 39.0 33.0 39.0 10 36.75382848783681 39.0 37.0 39.0 33.0 39.0 11 36.79100727335966 39.0 37.0 39.0 33.0 39.0 12 36.721057876574534 39.0 37.0 39.0 33.0 39.0 13 36.69101008654377 39.0 37.0 39.0 33.0 39.0 14 37.98592683334356 40.0 38.0 41.0 33.0 41.0 15 37.986079427269814 40.0 38.0 41.0 33.0 41.0 16 37.962512616704515 40.0 38.0 41.0 33.0 41.0 17 37.94097087246217 40.0 38.0 41.0 33.0 41.0 18 37.893377764592756 40.0 38.0 41.0 33.0 41.0 19 37.91760226350499 40.0 38.0 41.0 33.0 41.0 20 37.90391441782433 40.0 38.0 41.0 33.0 41.0 21 37.84988295449121 40.0 38.0 41.0 33.0 41.0 22 37.78891997599416 40.0 38.0 41.0 33.0 41.0 23 37.68321671406456 40.0 38.0 41.0 32.0 41.0 24 37.65347880052649 40.0 38.0 41.0 32.0 41.0 25 37.60015915802252 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19566754131344766 1101 2 0.29223079578485667 1101 3 0.2825610099556215 1101 4 0.37075619720268804 1101 5 0.008012035277282337 1101 6 0.1415453701241276 1101 7 0.268706917298303 1101 8 0.23387638736150507 1101 9 0.41042112627763316 1101 10 0.38287022356824707 1101 11 0.40245236655729855 1101 12 0.3951998412913369 1101 13 0.41393123057193293 1101 14 0.49422292459195916 1101 15 0.5597351321145183 1101 16 0.4568196194728884 1101 17 0.5517160823274452 1101 18 0.45069149853497237 1101 19 0.5552295978241091 1101 20 0.5658392234439873 1101 21 0.5714615032080204 1101 22 0.6287619489394771 1101 23 0.5961822877960827 1101 24 0.6486537425692589 1101 25 0.7104428835646104 1102 1 0.022492731531126964 1102 2 0.17193068043728132 1102 3 0.237838525379388 1102 4 0.18713032812748054 1102 5 -0.11500939662002452 1102 6 0.1511503377421448 1102 7 0.23405006311245558 1102 8 0.28378174149973034 1102 9 0.2996082499429349 1102 10 0.34243557352645126 1102 11 0.26366472647348616 1102 12 0.2625044911744041 1102 13 0.38441557795862025 1102 14 0.3747870893273486 1102 15 0.45495121701668495 1102 16 0.5179647484187839 1102 17 0.5333889462807946 1102 18 0.5656557963433642 1102 19 0.5318872562682984 1102 20 0.5018004841189239 1102 21 0.5811314530476963 1102 22 0.4820709494897599 1102 23 0.6016393062728582 1102 24 0.5305828646577879 1102 25 0.4585843589553633 1103 1 0.040072647262910266 1103 2 0.09066027011906286 1103 3 0.19056497689426521 1103 4 0.23826203842200755 1103 5 0.043939658710613116 1103 6 0.16205360056008544 1103 7 0.2081157598705019 1103 8 0.3153813567298229 1103 9 0.48924537766387743 1103 10 0.4802243763099767 1103 11 0.42987538027328753 1103 12 0.39273584245336224 1103 13 0.5293415535332002 1103 14 0.5543043692049636 1103 15 0.5298189362634176 1103 16 0.5491535818499784 1103 17 0.6300490262737952 1103 18 0.6293337356867355 1103 19 0.6015962192953594 1103 20 0.5745928860721392 1103 21 0.6178188390847197 1103 22 0.6713642567081877 1103 23 0.691346035110513 1103 24 0.7196316665656752 1103 25 0.663363592712102 1104 1 -0.06896766575833624 1104 2 0.025254722812132258 1104 3 0.07070557504562203 1104 4 0.09285960525051706 1104 5 -0.26455311714395435 1104 6 -0.0896049376539807 1104 7 0.12724033335815932 1104 8 0.10243857944320212 1104 9 0.2219459043293739 1104 10 0.2570182441215749 1104 11 0.09891889604357829 1104 12 0.11524343038931306 1104 13 0.17747289029640712 1104 14 0.10250914449746062 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-2.280891345193261 2314 15 -1.5566656045429 2314 16 -1.2340064820486774 2314 17 -0.9812376191514431 2314 18 -0.8379195306527976 2314 19 -0.7611170415976218 2314 20 -0.7016989288439817 2314 21 -0.5606866291092061 2314 22 -0.5328338102258172 2314 23 -0.5512241793129036 2314 24 -0.5650915089335555 2314 25 -0.6896896576985725 2315 1 -0.8563604218905354 2315 2 -0.7302107380831444 2315 3 -0.6210803732486916 2315 4 -0.45220553634267446 2315 5 -0.1789705580076557 2315 6 -0.46412716720271874 2315 7 -0.6127362850057878 2315 8 -0.8500402638109108 2315 9 -0.9326332907313741 2315 10 -0.7980576199508462 2315 11 -0.7180955933845325 2315 12 -0.7657258129232858 2315 13 -0.828799253690299 2315 14 -0.8489208962851365 2315 15 -1.0275926037128968 2315 16 -0.9279039779294251 2315 17 -1.0131978857113282 2315 18 -1.0057514457335657 2315 19 -0.9763109965473404 2315 20 -0.9180072420576764 2315 21 -0.7997879863540476 2315 22 -0.8005641479623904 2315 23 -0.9639550423601904 2315 24 -0.976490375739246 2315 25 -0.9658890923321053 2316 1 -0.4256653340320007 2316 2 -0.45658278921542816 2316 3 -0.4962119046757856 2316 4 -0.439640606633958 2316 5 -0.212272866272734 2316 6 -0.4316606408362347 2316 7 -1.0005105503247407 2316 8 -0.824245278948176 2316 9 -0.7252345364900421 2316 10 -0.8210817503943559 2316 11 -0.8177404585313823 2316 12 -0.8402908674753888 2316 13 -0.7634858744918844 2316 14 -0.9582368882773267 2316 15 -1.0274439063198955 2316 16 -1.0311609412923488 2316 17 -1.1435878875393897 2316 18 -1.1835429216053512 2316 19 -1.315749468820961 2316 20 -1.3060074516162246 2316 21 -1.3443249734372102 2316 22 -1.2261700842655117 2316 23 -1.0927527336492417 2316 24 -1.2157190665333815 2316 25 -1.2496353748972808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17899.0 3 1.0 4 432.0 5 3014.0 6 3757.0 7 3012.0 8 2436.0 9 2211.0 10 2039.0 11 1902.0 12 1808.0 13 1791.0 14 1792.0 15 1843.0 16 1870.0 17 2064.0 18 2261.0 19 2479.0 20 3182.0 21 4229.0 22 5679.0 23 6907.0 24 8560.0 25 10321.0 26 11955.0 27 13935.0 28 16001.0 29 19928.0 30 25758.0 31 33899.0 32 44616.0 33 59546.0 34 80649.0 35 111852.0 36 168796.0 37 315133.0 38 1352539.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.84042507788826 19.739490418676112 13.369809227092313 25.050275276343303 2 14.389282537149356 21.129853627638155 41.452675039204976 23.028188796007505 3 18.347960632035704 25.708781067913574 30.532251808343315 25.41100649170741 4 12.23554034296529 17.877715781262655 38.840925656753384 31.045818219018674 5 12.467576991187423 38.647970659708825 36.37116440549538 12.513287943608375 6 30.686439568769515 38.08465074755912 18.546261948603473 12.682647735067896 7 26.9969765903169 31.99003621952717 23.192945718456272 17.820041471699657 8 24.408019230875805 37.84333008925272 21.090062242866114 16.658588437005367 9 26.37141711005398 14.935711858850432 20.826919297611397 37.86595173348419 10 14.70450180443082 29.409770998224342 35.33887701093317 20.546850186411667 11 33.362068855256105 22.42926862127904 25.69207202298501 18.51659050047985 12 23.138523745855903 25.12568727233038 32.647311622576645 19.08847735923708 13 28.82377267591823 22.363496834431157 26.068512715567778 22.744217774082838 14 20.20992978184286 23.09739145855287 28.329692833928434 28.362985925675837 15 22.548326643088814 31.971851520908007 23.64552390241906 21.834297933584118 16 20.99381990547188 28.43337992809997 29.368770934073734 21.20402923235442 17 21.14635415845176 29.2311443633978 28.62048812428144 21.002013353869003 18 20.88687425589751 27.524108600918947 31.323299290222668 20.265717852960872 19 23.062102818298417 27.053431262017487 28.55049378511471 21.33397213456939 20 23.137872198587182 27.74314573207456 28.9860632123279 20.132918857010356 21 22.121776180037347 27.030324058188704 28.178361367909428 22.66953839386452 22 22.534409193403324 28.771584336091184 27.942396303542584 20.751610166962912 23 21.24171431031477 28.572889986330292 28.763720326351 21.421675377003943 24 22.411039411917905 27.52056953190169 29.380831834725946 20.68755922145446 25 21.86471562319514 27.846868946057175 28.90952838653166 21.37888704421603 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 426.0 1 426.0 2 655.5 3 885.0 4 885.0 5 885.0 6 2193.5 7 3502.0 8 3502.0 9 3502.0 10 4879.5 11 6257.0 12 6257.0 13 6257.0 14 9500.0 15 12743.0 16 12743.0 17 12743.0 18 23045.0 19 33347.0 20 33347.0 21 33347.0 22 51460.0 23 69573.0 24 69573.0 25 69573.0 26 99204.5 27 128836.0 28 128836.0 29 128836.0 30 164431.5 31 200027.0 32 200027.0 33 200027.0 34 222839.5 35 245652.0 36 245652.0 37 245652.0 38 258229.5 39 270807.0 40 270807.0 41 270807.0 42 280764.0 43 290721.0 44 290721.0 45 290721.0 46 301727.0 47 312733.0 48 312733.0 49 312733.0 50 293237.5 51 273742.0 52 273742.0 53 273742.0 54 236874.5 55 200007.0 56 200007.0 57 200007.0 58 168357.5 59 136708.0 60 136708.0 61 136708.0 62 110366.5 63 84025.0 64 84025.0 65 84025.0 66 64463.5 67 44902.0 68 44902.0 69 44902.0 70 32323.0 71 19744.0 72 19744.0 73 19744.0 74 13558.0 75 7372.0 76 7372.0 77 7372.0 78 5108.0 79 2844.0 80 2844.0 81 2844.0 82 1866.5 83 889.0 84 889.0 85 889.0 86 574.0 87 259.0 88 259.0 89 259.0 90 165.0 91 71.0 92 71.0 93 71.0 94 42.5 95 14.0 96 14.0 97 14.0 98 12.0 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12770150923065382 2 0.003964032162366757 3 0.001108224045392857 4 0.0036656641501456038 5 0.03942720161493818 6 0.02254809692357005 7 0.017049600698351645 8 0.012147840497575547 9 0.01402329657439423 10 0.020502144839767852 11 0.024167808989913456 12 0.025233409033560434 13 0.023272704953249995 14 0.011252736460912086 15 0.01670860868438461 16 0.01444953659185302 17 0.01103961645218269 18 0.006350976260135987 19 0.0014918400611057688 20 1.2787200523763735E-4 21 2.1312000872939556E-4 22 0.0028131841152280214 23 0.0014065920576140107 24 0.001236096050630494 25 4.262400174587911E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2346096.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.75569653280438 #Duplication Level Percentage of deduplicated Percentage of total 1 82.13923911237832 55.654013587337616 2 12.205020455693642 16.539193243452964 3 3.047031568558502 6.193612388553744 4 1.0433644693406792 2.827755454310301 5 0.49627869454571555 1.6812854311667913 6 0.2790416178424232 1.1343995507132398 7 0.1599424594008575 0.7585908919312421 8 0.11328327631917076 0.6140469834018846 9 0.08662352197453749 0.5282313360758527 >10 0.3809529432119485 4.581223263067608 >50 0.024658275707596107 1.153517751844165 >100 0.019228933745182038 2.731034594671777 >500 0.0026987160600127486 1.278230218955327 >1k 0.0021966293511700113 2.6243713351323557 >5k 4.393258702340022E-4 1.7004939693852024 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7582 0.3231751812372554 No Hit GTACATGGGAAGCAGTGGTATCAAC 5915 0.25212097032687497 No Hit GTCCTACAGTGGACATTTCTAAATT 5544 0.2363074656791538 No Hit CTGTAGGACGTGGAATATGGCAAGA 5497 0.2343041375970975 No Hit TATCAACGCAGAGTACTTTTTTTTT 5244 0.22352026515539006 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5163 0.22006772101397384 No Hit GGTATCAACGCAGAGTACTTTTTTT 5044 0.21499546480621426 No Hit CTTTAGGACGTGAAATATGGCGAGG 4953 0.2111166806473392 No Hit CCCATGTACTCTGCGTTGATACCAC 4066 0.1733091910987445 No Hit CTGAAGGACCTGGAATATGGCGAGA 2776 0.11832422884656042 No Hit GTCCTACAGTGTGCATTTCTCATTT 2720 0.11593728474879117 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2479 0.10566490032803433 No Hit GAGTACATGGGAAGCAGTGGTATCA 2463 0.10498291630010026 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.2624001745879114E-5 0.0 8 0.0 0.0 0.0 4.2624001745879114E-5 0.0 9 0.0 0.0 0.0 8.524800349175823E-5 4.2624001745879114E-5 10 0.0 0.0 0.0 8.524800349175823E-5 4.2624001745879114E-5 11 0.0 0.0 0.0 8.524800349175823E-5 4.2624001745879114E-5 12 0.0 0.0 0.0 8.524800349175823E-5 4.2624001745879114E-5 13 0.0 0.0 0.0 8.524800349175823E-5 4.2624001745879114E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCGC 25 0.0060358276 18.998444 6 AGTACGG 40 0.0052695973 14.254303 5 GCGTGCG 95 7.402923E-8 12.998658 9 CGTGCGC 110 2.75395E-9 12.953208 10 TAGGACC 965 0.0 12.604841 4 CTAGGAC 265 0.0 12.550692 3 GTACGGC 55 0.003071131 12.089918 6 GCGCCAC 135 4.7421054E-9 11.257617 13 GACCTCG 60 0.005882622 11.081953 7 CAGTACG 70 0.0014881397 10.860421 4 ACTTCGT 70 0.0014934437 10.855791 8 GGCGAGG 1355 0.0 10.3724375 19 TGTCCAC 930 0.0 10.316139 10 GGCGTGC 175 2.3646862E-10 10.313001 8 GTCCTAC 2795 0.0 10.210748 1 ATTTAGA 900 0.0 10.147214 1 GTATGAC 150 2.4356268E-8 10.136177 3 AACCGTG 75 0.00265117 10.132071 7 AGGACCT 2000 0.0 10.120555 5 TCCAACG 160 6.206392E-9 10.090773 18 >>END_MODULE